Background - Human tissue kallikrein (HK) releases kinins from kininogen. We investigated whether adenovirus-mediated HK gene delivery is angiogenic in the context of ischemia. Methods and Results - Hindlimb ischemia, caused by femoral artery excision, increased muscular capillary density (P1 receptor gene (P1 receptors blunted ischemia-induced angiogenesis (P2 receptor antagonism was ineffective. Intramuscular delivery of adenovirus containing the HK gene (Ad.CMV-cHK) enhanced the increase in capillary density caused by ischemia (969±32 versus 541±18 capillaries/mm 2 for control, P1 or B 2 receptors prevented HK-induced angiogenesis. Conclusions - HK gene delivery enhances the native angiogenic response to ischemia. Angiogenesis gene therapy with HK might be applicable to peripheral occlusive vascular disease.
|Number of pages||8|
|Publication status||Published - Jan 9 2001|
- Gene therapy
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine