TY - JOUR
T1 - Localisation of Bgl2p upon antifungal drug treatment in Candida albicans
AU - Angiolella, Letizia
AU - Vitali, Alberto
AU - Stringaro, Annarita
AU - Mignogna, Giuseppina
AU - Maras, Bruno
AU - Bonito, Mariantonietta
AU - Colone, Marisa
AU - Palamara, Anna Teresa
AU - Cassone, Antonio
PY - 2009/2
Y1 - 2009/2
N2 - Several proteins are covalently bound to the cell wall glucan (glucan-associated proteins (GAPs)) in Candida albicans and different drugs may cause their modulation. Proteomic analysis is a suitable approach to study differential GAP patterns between control and drug-treated cells. Since antimycotics induce variation in GAP content, we investigated the effect of a sublethal dose of micafungin and observed a clear increase in Bgl2p, an enzyme with glucanosyltransferase activity, with respect to a general decrease in cell wall protein content. Immunoelectron microscopy using mouse antiserum confirmed this increase of Bgl2p on the outer cell wall but also revealed a dramatic increase in the immature Bgl2p isoform in the cytoplasm of drug-treated cells. Since this increased expression of Bgl2p is clearly dependent upon micafungin treatment, this enzyme appears to be one of the survival strategies of C. albicans and thus could be considered the molecular basis of antifungal resistance and also as a potential valuable candidate for future vaccine development.
AB - Several proteins are covalently bound to the cell wall glucan (glucan-associated proteins (GAPs)) in Candida albicans and different drugs may cause their modulation. Proteomic analysis is a suitable approach to study differential GAP patterns between control and drug-treated cells. Since antimycotics induce variation in GAP content, we investigated the effect of a sublethal dose of micafungin and observed a clear increase in Bgl2p, an enzyme with glucanosyltransferase activity, with respect to a general decrease in cell wall protein content. Immunoelectron microscopy using mouse antiserum confirmed this increase of Bgl2p on the outer cell wall but also revealed a dramatic increase in the immature Bgl2p isoform in the cytoplasm of drug-treated cells. Since this increased expression of Bgl2p is clearly dependent upon micafungin treatment, this enzyme appears to be one of the survival strategies of C. albicans and thus could be considered the molecular basis of antifungal resistance and also as a potential valuable candidate for future vaccine development.
KW - Bgl2p
KW - Candida albicans
KW - Cell wall proteins
KW - Glucanosyltransferase
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U2 - 10.1016/j.ijantimicag.2008.08.021
DO - 10.1016/j.ijantimicag.2008.08.021
M3 - Article
C2 - 19013773
AN - SCOPUS:58149181472
VL - 33
SP - 143
EP - 148
JO - International Journal of Antimicrobial Agents
JF - International Journal of Antimicrobial Agents
SN - 0924-8579
IS - 2
ER -