Localization and age-dependent expression of the inward rectifier K+ channel subunit Kir 5.1 in a mammalian reproductive system

Lorena Salvatore, Maria Cristina D'Adamo, Roman Polishchuk, Mario Salmona, Mauro Pessia

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Kir 5.1 is a member of the inward rectifier potassium channel superfamily which does not form functional channels when expressed by itself in Xenopus laevis oocytes. rt-PCR reveals high levels of Kir 5.1 mRNA expression in testis but the function of this channel remains unknown. To determine the cell-specific expression of this channel in the testis we raised a polyclonal antibody against an external epitope of Kir 5.1 and tested its specificity in Xenopus oocytes expressing several cloned Kir subunits. Strong immunoreactivity for Kir 5.1 was found in seminiferous tubules of rat testis and, particularly, in spermatogonia, primary and secondary spermatocytes, spermatids and in the head and body of spermatozoa. The intensity of Kir 5.1 immunofluorescence, quantified using laser scanning microscopy, increased with age at every stage in the development of sperm from spermatogonia and reached a peak in 60-day-old rats. In contrast, the immunofluorescence decreased in 90-day-old animals and was detected mostly in spermatozoa. The results demonstrate that Kir 5.1 expression in the testis is localised to cells involved in spermatogenesis, showing a temporal pattern of expression during sexual maturity. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)146-152
Number of pages7
JournalFEBS Letters
Volume449
Issue number2-3
DOIs
Publication statusPublished - Apr 23 1999

Fingerprint

Inwardly Rectifying Potassium Channel
Testis
Rats
Spermatozoa
Spermatogonia
Oocytes
Fluorescent Antibody Technique
Epitopes
Microscopic examination
Animals
Scanning
Messenger RNA
Seminiferous Tubules
Spermatocytes
Spermatids
Antibodies
Lasers
Xenopus laevis
Spermatogenesis
Xenopus

Keywords

  • Confocal microscopy
  • Immunofluorescence
  • Immunohistochemistry
  • Potassium channel
  • Spermatozoon
  • Testis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Localization and age-dependent expression of the inward rectifier K+ channel subunit Kir 5.1 in a mammalian reproductive system. / Salvatore, Lorena; D'Adamo, Maria Cristina; Polishchuk, Roman; Salmona, Mario; Pessia, Mauro.

In: FEBS Letters, Vol. 449, No. 2-3, 23.04.1999, p. 146-152.

Research output: Contribution to journalArticle

Salvatore, Lorena ; D'Adamo, Maria Cristina ; Polishchuk, Roman ; Salmona, Mario ; Pessia, Mauro. / Localization and age-dependent expression of the inward rectifier K+ channel subunit Kir 5.1 in a mammalian reproductive system. In: FEBS Letters. 1999 ; Vol. 449, No. 2-3. pp. 146-152.
@article{e40ae81fcb5543d3b8dbc00b686a62b1,
title = "Localization and age-dependent expression of the inward rectifier K+ channel subunit Kir 5.1 in a mammalian reproductive system",
abstract = "Kir 5.1 is a member of the inward rectifier potassium channel superfamily which does not form functional channels when expressed by itself in Xenopus laevis oocytes. rt-PCR reveals high levels of Kir 5.1 mRNA expression in testis but the function of this channel remains unknown. To determine the cell-specific expression of this channel in the testis we raised a polyclonal antibody against an external epitope of Kir 5.1 and tested its specificity in Xenopus oocytes expressing several cloned Kir subunits. Strong immunoreactivity for Kir 5.1 was found in seminiferous tubules of rat testis and, particularly, in spermatogonia, primary and secondary spermatocytes, spermatids and in the head and body of spermatozoa. The intensity of Kir 5.1 immunofluorescence, quantified using laser scanning microscopy, increased with age at every stage in the development of sperm from spermatogonia and reached a peak in 60-day-old rats. In contrast, the immunofluorescence decreased in 90-day-old animals and was detected mostly in spermatozoa. The results demonstrate that Kir 5.1 expression in the testis is localised to cells involved in spermatogenesis, showing a temporal pattern of expression during sexual maturity. Copyright (C) 1999 Federation of European Biochemical Societies.",
keywords = "Confocal microscopy, Immunofluorescence, Immunohistochemistry, Potassium channel, Spermatozoon, Testis",
author = "Lorena Salvatore and D'Adamo, {Maria Cristina} and Roman Polishchuk and Mario Salmona and Mauro Pessia",
year = "1999",
month = "4",
day = "23",
doi = "10.1016/S0014-5793(99)00420-2",
language = "English",
volume = "449",
pages = "146--152",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "2-3",

}

TY - JOUR

T1 - Localization and age-dependent expression of the inward rectifier K+ channel subunit Kir 5.1 in a mammalian reproductive system

AU - Salvatore, Lorena

AU - D'Adamo, Maria Cristina

AU - Polishchuk, Roman

AU - Salmona, Mario

AU - Pessia, Mauro

PY - 1999/4/23

Y1 - 1999/4/23

N2 - Kir 5.1 is a member of the inward rectifier potassium channel superfamily which does not form functional channels when expressed by itself in Xenopus laevis oocytes. rt-PCR reveals high levels of Kir 5.1 mRNA expression in testis but the function of this channel remains unknown. To determine the cell-specific expression of this channel in the testis we raised a polyclonal antibody against an external epitope of Kir 5.1 and tested its specificity in Xenopus oocytes expressing several cloned Kir subunits. Strong immunoreactivity for Kir 5.1 was found in seminiferous tubules of rat testis and, particularly, in spermatogonia, primary and secondary spermatocytes, spermatids and in the head and body of spermatozoa. The intensity of Kir 5.1 immunofluorescence, quantified using laser scanning microscopy, increased with age at every stage in the development of sperm from spermatogonia and reached a peak in 60-day-old rats. In contrast, the immunofluorescence decreased in 90-day-old animals and was detected mostly in spermatozoa. The results demonstrate that Kir 5.1 expression in the testis is localised to cells involved in spermatogenesis, showing a temporal pattern of expression during sexual maturity. Copyright (C) 1999 Federation of European Biochemical Societies.

AB - Kir 5.1 is a member of the inward rectifier potassium channel superfamily which does not form functional channels when expressed by itself in Xenopus laevis oocytes. rt-PCR reveals high levels of Kir 5.1 mRNA expression in testis but the function of this channel remains unknown. To determine the cell-specific expression of this channel in the testis we raised a polyclonal antibody against an external epitope of Kir 5.1 and tested its specificity in Xenopus oocytes expressing several cloned Kir subunits. Strong immunoreactivity for Kir 5.1 was found in seminiferous tubules of rat testis and, particularly, in spermatogonia, primary and secondary spermatocytes, spermatids and in the head and body of spermatozoa. The intensity of Kir 5.1 immunofluorescence, quantified using laser scanning microscopy, increased with age at every stage in the development of sperm from spermatogonia and reached a peak in 60-day-old rats. In contrast, the immunofluorescence decreased in 90-day-old animals and was detected mostly in spermatozoa. The results demonstrate that Kir 5.1 expression in the testis is localised to cells involved in spermatogenesis, showing a temporal pattern of expression during sexual maturity. Copyright (C) 1999 Federation of European Biochemical Societies.

KW - Confocal microscopy

KW - Immunofluorescence

KW - Immunohistochemistry

KW - Potassium channel

KW - Spermatozoon

KW - Testis

UR - http://www.scopus.com/inward/record.url?scp=0032910451&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032910451&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(99)00420-2

DO - 10.1016/S0014-5793(99)00420-2

M3 - Article

C2 - 10338121

AN - SCOPUS:0032910451

VL - 449

SP - 146

EP - 152

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 2-3

ER -