Localization of amplified CAD genes on rearranged chromosomes of Chinese hamster cells

Laura Ottaggio, Cinzia Agnese, Stefania Bonatti, Pasqua Cavolina, Aldo Di Leonardo, Mariangela Miele, Angelo Abbondandolo

Research output: Contribution to journalArticle

Abstract

Chinese hamster cell lines carrying an amplified CAD region were selected from V79,B7 cells by their resistance to N-phosphonacetyl-L-aspartate (PALA). In one of the selected cell lines, SP PALAinf1 supRL, an acrocentric chromosome with abnormally elongated q arms was identified as a marker for the PALA-resistant phenotype. The marker chromosome carried a homogeneously staining region close to a telomeric nucleolar organizer region. In the same region, localization of amplified CAD sequences was demonstrated by in situ hybridization. The marker chromosome was found to undergo extensive rearrangements. In particular, dicentric chromosomes, occurring with an unusually high incidence, were found to originate from end-fusion of two homologous marker chromosomes.

Original languageEnglish
Pages (from-to)25-31
Number of pages7
JournalCytotechnology
Volume1
Issue number1
DOIs
Publication statusPublished - Oct 1987

Keywords

  • CAD gene
  • chromosome rearrangements
  • gene amplification
  • methotrexate-resistance
  • PALA-resistance

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Biotechnology

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  • Cite this

    Ottaggio, L., Agnese, C., Bonatti, S., Cavolina, P., Di Leonardo, A., Miele, M., & Abbondandolo, A. (1987). Localization of amplified CAD genes on rearranged chromosomes of Chinese hamster cells. Cytotechnology, 1(1), 25-31. https://doi.org/10.1007/BF00351118