Localization of brain and atrial natriuretic peptide in human and porcine heart

Angela Pucci, John Wharton, Eloisa Arbustini, Maurizia Grasso, Marta Diegoli, Philip Needleman, Mario Viganò, Gonzalo Moscoso, Julia M. Polak

Research output: Contribution to journalArticlepeer-review

Abstract

We have compared the localization of brain and atrial natriuretic peptide-like immunoreactivity in human and porcine hearts, using immunohistochemical techniques at both the light and ultrastructural level and specific antisera to amino-(cardiodilatin) and carboxy-terminal regions of the atrial natriuretic precursor molecule and to brain natriuretic peptide. Atrial myocardial cells in human fetal, normal adult and failing explanted hearts, displayed immunoreactivity for both brain and atrial natriuretic peptide-like sequences. At the subcellular level, brain natriuretic peptide-, cardiodilatin- and alpha-atrial natriuretic peptide-like immunoreactivity were co-localized to secretory granules in atrial myocardial cells. Immunoreactivity was also detected in the left (64%) and right ventricular free walls (23%) of 22 failing explanted hearts, but not in donor cardiac tissues. A gradient of natriuretic peptide immunostaining was observed across ventricular free walls and immunoreactivity for both natriuretic peptide sequences co-localized to secretory granules in a subpopulation of myocardial cells, concentrated in subendocardial regions of the ventricular walls. Brain and atrial natriuretic peptide-like immunoreactivity were also demonstrated in porcine atrial myocardium and cells of the ventricular conduction system. The parallel distribution of cardiac brain and atrial natriuretic peptide-like immunoreactivity suggests a dual regulation and co-storage of the natriuretic peptides in human and porcine hearts.

Original languageEnglish
Pages (from-to)237-247
Number of pages11
JournalInternational Journal of Cardiology
Volume34
Issue number3
DOIs
Publication statusPublished - 1992

Keywords

  • Atrial natriuretic peptide
  • Brain natriuretic peptide
  • Conduction system
  • Electron microscopy
  • Immunohistochemistry

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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