Localization of dystrophin COOH-terminal domain by the fracture-label technique

Stefano Squarzoni, Patrizia Sabatelli, Maria Cristina Maltarello, Amelia Cataldi, Roberto Di Primio, Nadir Mario Maraldi

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The precise localization of dystrophin in the skeletal muscle cell should contribute to a better understanding of the yet unclear functional role of this protein, both in normal and in Duchenne muscular dystrophy. Immunocytochemical studies did not give conclusive results on the localization of dystrophin with respect to the sarcolemma and to the cytoskeletal components. To improve the reliability of the electron microscopic immunocytochemical localization of dystrophin, a mAb against the COOH-terminus of the molecule has been used in association with the fracture-label technique, which, causing a partition of the membrane in protoplasmic and exoplasmic halves, allows a more precise dystrophin localization. The results obtained indicate that dystrophin is associated with the protoplasmic half of the plasmalemma, and the observation that it does not randomly follow the partition of the membrane is consistent with a stable association with the cytoskeleton.

Original languageEnglish
Pages (from-to)1401-1409
Number of pages9
JournalJournal of Cell Biology
Volume118
Issue number6
Publication statusPublished - Sep 1992

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Dystrophin
Sarcolemma
Membranes
Duchenne Muscular Dystrophy
Cytoskeleton
Muscle Cells
Skeletal Muscle
Electrons
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Squarzoni, S., Sabatelli, P., Maltarello, M. C., Cataldi, A., Di Primio, R., & Maraldi, N. M. (1992). Localization of dystrophin COOH-terminal domain by the fracture-label technique. Journal of Cell Biology, 118(6), 1401-1409.

Localization of dystrophin COOH-terminal domain by the fracture-label technique. / Squarzoni, Stefano; Sabatelli, Patrizia; Maltarello, Maria Cristina; Cataldi, Amelia; Di Primio, Roberto; Maraldi, Nadir Mario.

In: Journal of Cell Biology, Vol. 118, No. 6, 09.1992, p. 1401-1409.

Research output: Contribution to journalArticle

Squarzoni, S, Sabatelli, P, Maltarello, MC, Cataldi, A, Di Primio, R & Maraldi, NM 1992, 'Localization of dystrophin COOH-terminal domain by the fracture-label technique', Journal of Cell Biology, vol. 118, no. 6, pp. 1401-1409.
Squarzoni, Stefano ; Sabatelli, Patrizia ; Maltarello, Maria Cristina ; Cataldi, Amelia ; Di Primio, Roberto ; Maraldi, Nadir Mario. / Localization of dystrophin COOH-terminal domain by the fracture-label technique. In: Journal of Cell Biology. 1992 ; Vol. 118, No. 6. pp. 1401-1409.
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