Localization of Epstein-Barr virus envelope glycoproteins on the inner nuclear membrane of virus-producing cells

M. R. Torrisi, M. Cirone, A. Pavan, C. Zompetta, G. Barile, L. Frati, A. Faggioni

Research output: Contribution to journalArticle

Abstract

Epstein-Barr virus-producing cells were used as a model to analyze, with a fracture-immunolabel technique, the distribution, behavior on fracture, and extent of glycosylation of viral transmembrane glycoproteins at the inner nuclear membrane. Surface and fracture immunolabeling with two monoclonal antibodies directed against the carbohydrate or polypeptide portions of the major viral envelope glycoproteins gp350/220 showed the following. (i) The glycoproteins present on the inner and outer nuclear membranes were labeled only with the monoclonal antibody directed against the polypeptide chain, whereas over the surface of virus-producing cells and on mature virions the labeling was dense and uniformly distributed with both monoclonal antibodies. (ii) The glycoproteins were nonuniformly distributed only over the inner nuclear membranes; at the sites of viral budding, the glycoproteins showed a preferential partition with the protoplasmic face. Since fully glycosylated glycoproteins were not present on the nuclear membranes, our observations support the proposed model of herpesvirus maturation. The peculiar distribution and partition on fracture of the envelope glycoproteins on the inner membrane are similar to those of Sindbis virus envelope glycoproteins on the plasma membrane of infected cells. Therefore, our results suggest that inner nuclear membranes may behave like plasma membranes during viral assembly.

Original languageEnglish
Pages (from-to)828-832
Number of pages5
JournalJournal of Virology
Volume63
Issue number2
Publication statusPublished - 1989

    Fingerprint

ASJC Scopus subject areas

  • Immunology

Cite this

Torrisi, M. R., Cirone, M., Pavan, A., Zompetta, C., Barile, G., Frati, L., & Faggioni, A. (1989). Localization of Epstein-Barr virus envelope glycoproteins on the inner nuclear membrane of virus-producing cells. Journal of Virology, 63(2), 828-832.