TY - JOUR
T1 - Localization of glucose-6-phosphate dehydrogenase in mouse and man by in situ hybridization
T2 - Evidence for a single locus and transposition of homologous X-linked genes
AU - Martin-DeLeon, P. A.
AU - Wolf, S. F.
AU - Persico, G.
AU - Toniolo, D.
AU - Martini, G.
AU - Migeon, B. R.
PY - 1985
Y1 - 1985
N2 - By hybridizing a tritiatcd human genomic probe (pGD3) to metaphase chromosomes in situ, we have localized the gene for glucose-6-phosphate dehydrogenase (G6PD) in both the human and mouse complement. The locus on the intact human X chromosome is close to the telomere on the long arm. confirming the assignment based on studies of an X/autosome translocation in human-mouse hybrids. Although the signahbackground ratio was reduced for the heterologous hybridization of the human probe to mouse metaphases, 20% of the grains were on the X chromosome and 93% of these were in the A region, relatively close to the centromere. The location of G6PD in mouse and man reflects inirachromosomal transposition of these homologous X loci. Genomic DNAs from mouse and man and from hybrids with human X/autosome translocations were digested with several restriction enzymes including EcoRl, Pstl, and Hpcill, and Southern blots were probed with 32P-pGD3. The results of the analysis also confirm the human G6PD assignment and are consistent with a single copy of the locus in the haploid genome of both species.
AB - By hybridizing a tritiatcd human genomic probe (pGD3) to metaphase chromosomes in situ, we have localized the gene for glucose-6-phosphate dehydrogenase (G6PD) in both the human and mouse complement. The locus on the intact human X chromosome is close to the telomere on the long arm. confirming the assignment based on studies of an X/autosome translocation in human-mouse hybrids. Although the signahbackground ratio was reduced for the heterologous hybridization of the human probe to mouse metaphases, 20% of the grains were on the X chromosome and 93% of these were in the A region, relatively close to the centromere. The location of G6PD in mouse and man reflects inirachromosomal transposition of these homologous X loci. Genomic DNAs from mouse and man and from hybrids with human X/autosome translocations were digested with several restriction enzymes including EcoRl, Pstl, and Hpcill, and Southern blots were probed with 32P-pGD3. The results of the analysis also confirm the human G6PD assignment and are consistent with a single copy of the locus in the haploid genome of both species.
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U2 - 10.1159/000132113
DO - 10.1159/000132113
M3 - Article
C2 - 4006523
AN - SCOPUS:0021796122
VL - 39
SP - 87
EP - 92
JO - Cytogenetic and Genome Research
JF - Cytogenetic and Genome Research
SN - 1424-8581
IS - 2
ER -