Localization of HCN1 channels to presynaptic compartments: Novel plasticity that may contribute to hippocampal maturation

Roland A. Bender, Timo Kirschstein, Oliver Kretz, Amy L. Brewster, Cristina Richichi, Christiane Rüschenschmidt, Ryuichi Shigemoto, Heinz Beck, Michael Frotscher, Tallie Z. Baram

Research output: Contribution to journalArticle

Abstract

Increasing evidence supports roles for the current mediated by hyperpolarization-activated cyclic nucleotide-gated (HCN) channels, I h, in hippocampal maturation and specifically in the evolving changes of intrinsic properties as well as network responses of hippocampal neurons. Here, we describe a novel developmental plasticity of HCN channel expression in axonal and presynaptic compartments: HCN1 channels were localized to axon terminals of the perforant path (the major hippocampal afferent pathway) of immature rats, where they modulated synaptic efficacy. However, presynaptic expression and functions of the channels disappeared with maturation. This was a result of altered channel transport to the axons, because HCN1 mRNA and protein levels in entorhinal cortex neurons, where the perforant path axons originate, were stable through adulthood. Blocking action potential firing in vitro increased presynaptic expression of HCN1 channels in the perforant path, suggesting that network activity contributed to regulating this expression. These findings support a novel developmentally regulated axonal transport of functional ion channels and suggest a role for HCN1 channel-mediated presynaptic Ih in hippocampal maturation.

Original languageEnglish
Pages (from-to)4697-4706
Number of pages10
JournalJournal of Neuroscience
Volume27
Issue number17
DOIs
Publication statusPublished - Apr 25 2007

Keywords

  • Development
  • Entorhinal cortex
  • Ion channel
  • Neuronal activity
  • Perforant path
  • Synaptic plasticity

ASJC Scopus subject areas

  • Neuroscience(all)

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    Bender, R. A., Kirschstein, T., Kretz, O., Brewster, A. L., Richichi, C., Rüschenschmidt, C., Shigemoto, R., Beck, H., Frotscher, M., & Baram, T. Z. (2007). Localization of HCN1 channels to presynaptic compartments: Novel plasticity that may contribute to hippocampal maturation. Journal of Neuroscience, 27(17), 4697-4706. https://doi.org/10.1523/JNEUROSCI.4699-06.2007