Localization of magic-F1 transgene, involved in muscular hypertrophy, during early myogenesis

Flavio Ronzoni, Matilde Bongio, Silvio Conte, Luigi Vercesi, Marco Cassano, Carla Tribioli, Daniela Galli, Riccardo Bellazzi, Giovanni Magenes, Maria Gabriella Cusella De Angelis, Maurilio Sampaolesi

Research output: Contribution to journalArticle

Abstract

We recently showed that Magic-F1 (Met-activating genetically improved chimeric factor 1), a human recombinant protein derived from hepatocyte growth factor/scatter factor (HGF/SF) induces muscle cell hypertrophy but not progenitor cell proliferation, both in vitro and in vivo. Here, we examined the temporal and spatial expression pattern of Magic-F1 in comparison with Pax3 (paired box gene 3) transcription factor during embryogenesis. Ranging from 9.5 to 17.5dpc (days post coitum) mouse embryos were analyzed by in situ hybridization using whole mounts during early stages of development (9.5-10.5-11.5dpc) and cryostat sections for later stages (11.5-13.5-15.5-17. 5dpc). We found that Magic-F1 is expressed in developing organs and tissues of mesenchymal origin, where Pax3 signal appears to be downregulated respect to the wt embryos. These data suggest that Magic-F1 could be responsible of muscular hypertrophy, cooperating with Pax3 signal pathway in skeletal muscle precursor cells.

Original languageEnglish
Article number492075
JournalJournal of Biomedicine and Biotechnology
Volume2011
DOIs
Publication statusPublished - 2011

Fingerprint

Magic
Hepatocyte Growth Factor
Muscle Development
Transgenes
Muscle Cells
Hypertrophy
Embryonic Structures
Recombinant Proteins
Embryonic Development
In Situ Hybridization
Signal Transduction
Skeletal Muscle
Stem Cells
Down-Regulation
Cell Proliferation
Genes
In Vitro Techniques
PAX3 Transcription Factor

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Medicine
  • Genetics
  • Molecular Biology
  • Health, Toxicology and Mutagenesis
  • Medicine(all)

Cite this

Localization of magic-F1 transgene, involved in muscular hypertrophy, during early myogenesis. / Ronzoni, Flavio; Bongio, Matilde; Conte, Silvio; Vercesi, Luigi; Cassano, Marco; Tribioli, Carla; Galli, Daniela; Bellazzi, Riccardo; Magenes, Giovanni; Cusella De Angelis, Maria Gabriella; Sampaolesi, Maurilio.

In: Journal of Biomedicine and Biotechnology, Vol. 2011, 492075, 2011.

Research output: Contribution to journalArticle

Ronzoni, F, Bongio, M, Conte, S, Vercesi, L, Cassano, M, Tribioli, C, Galli, D, Bellazzi, R, Magenes, G, Cusella De Angelis, MG & Sampaolesi, M 2011, 'Localization of magic-F1 transgene, involved in muscular hypertrophy, during early myogenesis', Journal of Biomedicine and Biotechnology, vol. 2011, 492075. https://doi.org/10.1155/2011/492075
Ronzoni, Flavio ; Bongio, Matilde ; Conte, Silvio ; Vercesi, Luigi ; Cassano, Marco ; Tribioli, Carla ; Galli, Daniela ; Bellazzi, Riccardo ; Magenes, Giovanni ; Cusella De Angelis, Maria Gabriella ; Sampaolesi, Maurilio. / Localization of magic-F1 transgene, involved in muscular hypertrophy, during early myogenesis. In: Journal of Biomedicine and Biotechnology. 2011 ; Vol. 2011.
@article{dc0ae6cf40e74005b37025691462c74d,
title = "Localization of magic-F1 transgene, involved in muscular hypertrophy, during early myogenesis",
abstract = "We recently showed that Magic-F1 (Met-activating genetically improved chimeric factor 1), a human recombinant protein derived from hepatocyte growth factor/scatter factor (HGF/SF) induces muscle cell hypertrophy but not progenitor cell proliferation, both in vitro and in vivo. Here, we examined the temporal and spatial expression pattern of Magic-F1 in comparison with Pax3 (paired box gene 3) transcription factor during embryogenesis. Ranging from 9.5 to 17.5dpc (days post coitum) mouse embryos were analyzed by in situ hybridization using whole mounts during early stages of development (9.5-10.5-11.5dpc) and cryostat sections for later stages (11.5-13.5-15.5-17. 5dpc). We found that Magic-F1 is expressed in developing organs and tissues of mesenchymal origin, where Pax3 signal appears to be downregulated respect to the wt embryos. These data suggest that Magic-F1 could be responsible of muscular hypertrophy, cooperating with Pax3 signal pathway in skeletal muscle precursor cells.",
author = "Flavio Ronzoni and Matilde Bongio and Silvio Conte and Luigi Vercesi and Marco Cassano and Carla Tribioli and Daniela Galli and Riccardo Bellazzi and Giovanni Magenes and {Cusella De Angelis}, {Maria Gabriella} and Maurilio Sampaolesi",
year = "2011",
doi = "10.1155/2011/492075",
language = "English",
volume = "2011",
journal = "Journal of Biomedicine and Biotechnology",
issn = "1110-7243",
publisher = "Hindawi Publishing Corporation",

}

TY - JOUR

T1 - Localization of magic-F1 transgene, involved in muscular hypertrophy, during early myogenesis

AU - Ronzoni, Flavio

AU - Bongio, Matilde

AU - Conte, Silvio

AU - Vercesi, Luigi

AU - Cassano, Marco

AU - Tribioli, Carla

AU - Galli, Daniela

AU - Bellazzi, Riccardo

AU - Magenes, Giovanni

AU - Cusella De Angelis, Maria Gabriella

AU - Sampaolesi, Maurilio

PY - 2011

Y1 - 2011

N2 - We recently showed that Magic-F1 (Met-activating genetically improved chimeric factor 1), a human recombinant protein derived from hepatocyte growth factor/scatter factor (HGF/SF) induces muscle cell hypertrophy but not progenitor cell proliferation, both in vitro and in vivo. Here, we examined the temporal and spatial expression pattern of Magic-F1 in comparison with Pax3 (paired box gene 3) transcription factor during embryogenesis. Ranging from 9.5 to 17.5dpc (days post coitum) mouse embryos were analyzed by in situ hybridization using whole mounts during early stages of development (9.5-10.5-11.5dpc) and cryostat sections for later stages (11.5-13.5-15.5-17. 5dpc). We found that Magic-F1 is expressed in developing organs and tissues of mesenchymal origin, where Pax3 signal appears to be downregulated respect to the wt embryos. These data suggest that Magic-F1 could be responsible of muscular hypertrophy, cooperating with Pax3 signal pathway in skeletal muscle precursor cells.

AB - We recently showed that Magic-F1 (Met-activating genetically improved chimeric factor 1), a human recombinant protein derived from hepatocyte growth factor/scatter factor (HGF/SF) induces muscle cell hypertrophy but not progenitor cell proliferation, both in vitro and in vivo. Here, we examined the temporal and spatial expression pattern of Magic-F1 in comparison with Pax3 (paired box gene 3) transcription factor during embryogenesis. Ranging from 9.5 to 17.5dpc (days post coitum) mouse embryos were analyzed by in situ hybridization using whole mounts during early stages of development (9.5-10.5-11.5dpc) and cryostat sections for later stages (11.5-13.5-15.5-17. 5dpc). We found that Magic-F1 is expressed in developing organs and tissues of mesenchymal origin, where Pax3 signal appears to be downregulated respect to the wt embryos. These data suggest that Magic-F1 could be responsible of muscular hypertrophy, cooperating with Pax3 signal pathway in skeletal muscle precursor cells.

UR - http://www.scopus.com/inward/record.url?scp=84855535726&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84855535726&partnerID=8YFLogxK

U2 - 10.1155/2011/492075

DO - 10.1155/2011/492075

M3 - Article

C2 - 22187527

AN - SCOPUS:84855535726

VL - 2011

JO - Journal of Biomedicine and Biotechnology

JF - Journal of Biomedicine and Biotechnology

SN - 1110-7243

M1 - 492075

ER -