Localization of magic-F1 transgene, involved in muscular hypertrophy, during early myogenesis

Flavio Ronzoni, Matilde Bongio, Silvio Conte, Luigi Vercesi, Marco Cassano, Carla Tribioli, Daniela Galli, Riccardo Bellazzi, Giovanni Magenes, Maria Gabriella Cusella De Angelis, Maurilio Sampaolesi

Research output: Contribution to journalArticle

Abstract

We recently showed that Magic-F1 (Met-activating genetically improved chimeric factor 1), a human recombinant protein derived from hepatocyte growth factor/scatter factor (HGF/SF) induces muscle cell hypertrophy but not progenitor cell proliferation, both in vitro and in vivo. Here, we examined the temporal and spatial expression pattern of Magic-F1 in comparison with Pax3 (paired box gene 3) transcription factor during embryogenesis. Ranging from 9.5 to 17.5dpc (days post coitum) mouse embryos were analyzed by in situ hybridization using whole mounts during early stages of development (9.5-10.5-11.5dpc) and cryostat sections for later stages (11.5-13.5-15.5-17. 5dpc). We found that Magic-F1 is expressed in developing organs and tissues of mesenchymal origin, where Pax3 signal appears to be downregulated respect to the wt embryos. These data suggest that Magic-F1 could be responsible of muscular hypertrophy, cooperating with Pax3 signal pathway in skeletal muscle precursor cells.

Original languageEnglish
Article number492075
JournalJournal of Biomedicine and Biotechnology
Volume2011
DOIs
Publication statusPublished - 2011

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Medicine
  • Genetics
  • Molecular Biology
  • Health, Toxicology and Mutagenesis
  • Medicine(all)

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    Ronzoni, F., Bongio, M., Conte, S., Vercesi, L., Cassano, M., Tribioli, C., Galli, D., Bellazzi, R., Magenes, G., Cusella De Angelis, M. G., & Sampaolesi, M. (2011). Localization of magic-F1 transgene, involved in muscular hypertrophy, during early myogenesis. Journal of Biomedicine and Biotechnology, 2011, [492075]. https://doi.org/10.1155/2011/492075