Localization of neuroglobin in the brain of R6/2 mouse model of Huntington’s disease

A. Cardinale, F. R. Fusco, E. Paldino, C. Giampà, M. Marino, M. T. Nuzzo, V. D’Angelo, D. Laurenti, G. Straccia, D. Fasano, D. Sarnataro, T. Squillaro, S. Paladino, Mariarosa A.B. Melone

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Neuroglobin (Ngb) is expressed in the central and peripheral nervous system, cerebrospinal fluid, retina, and endocrine tissues where it is involved in binding O2 and other gasotransmitters. Several studies have highlighted its endogenous neuroprotective function. Huntington’s disease (HD), a dominant hereditary disease, is characterized by the gradual loss of neurons in discrete areas of the central nervous system. We analyzed the expression of Ngb in the brain tissue of a mouse model of HD, in order to define the role of Ngb with respect to individual cell type vulnerability in HD and to gender and age of mice. Our results showed different expressions of Ngb among neurons of a specific region and between different brain regions. We evidenced a decreased intensity of Ngb at 13 weeks of age, compared to 7 weeks of age. The double immunofluorescence and fluorescence resonance energy transfer (FRET) experiments showed that the co-localization between Ngb and huntingtin at the subcellular level was not close enough to account for a direct interaction. We also observed a different expression of Ngb in the striatum, depending on the sex and age of animals. These findings provide the first experimental evidence for an adaptive response of Ngb in HD, suggesting that Ngb may exert neuroprotective effects in HD beyond its role in reducing sensitivity to oxidative stress.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalNeurological Sciences
DOIs
Publication statusAccepted/In press - Nov 3 2017

Fingerprint

Huntington Disease
Brain
Gasotransmitters
Central Nervous System
neuroglobin
Neurons
Fluorescence Resonance Energy Transfer
Inborn Genetic Diseases
Peripheral Nervous System
Neuroprotective Agents
Fluorescent Antibody Technique
Cerebrospinal Fluid
Retina
Oxidative Stress

Keywords

  • Brain
  • Huntington’s disease
  • Immunofluorescence
  • Neuroglobin
  • Neurological disease
  • R6/2 transgenic mouse

ASJC Scopus subject areas

  • Dermatology
  • Clinical Neurology
  • Psychiatry and Mental health

Cite this

Localization of neuroglobin in the brain of R6/2 mouse model of Huntington’s disease. / Cardinale, A.; Fusco, F. R.; Paldino, E.; Giampà, C.; Marino, M.; Nuzzo, M. T.; D’Angelo, V.; Laurenti, D.; Straccia, G.; Fasano, D.; Sarnataro, D.; Squillaro, T.; Paladino, S.; Melone, Mariarosa A.B.

In: Neurological Sciences, 03.11.2017, p. 1-11.

Research output: Contribution to journalArticle

Cardinale, A, Fusco, FR, Paldino, E, Giampà, C, Marino, M, Nuzzo, MT, D’Angelo, V, Laurenti, D, Straccia, G, Fasano, D, Sarnataro, D, Squillaro, T, Paladino, S & Melone, MAB 2017, 'Localization of neuroglobin in the brain of R6/2 mouse model of Huntington’s disease', Neurological Sciences, pp. 1-11. https://doi.org/10.1007/s10072-017-3168-2
Cardinale, A. ; Fusco, F. R. ; Paldino, E. ; Giampà, C. ; Marino, M. ; Nuzzo, M. T. ; D’Angelo, V. ; Laurenti, D. ; Straccia, G. ; Fasano, D. ; Sarnataro, D. ; Squillaro, T. ; Paladino, S. ; Melone, Mariarosa A.B. / Localization of neuroglobin in the brain of R6/2 mouse model of Huntington’s disease. In: Neurological Sciences. 2017 ; pp. 1-11.
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AU - Marino, M.

AU - Nuzzo, M. T.

AU - D’Angelo, V.

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AU - Straccia, G.

AU - Fasano, D.

AU - Sarnataro, D.

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AU - Paladino, S.

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