Localization of the cellular-fibronectin-specific epitope recognized by the monoclonal antibody IST-9 using fusion proteins expressed in E. coli

Barbara Carnemolla, Laura Borsi, Luciano Zardi, Raymond J. Owens, Francisco E. Baralle

Research output: Contribution to journalArticle

Abstract

Here we report on a monoclonal antibody (IST-9) which distinguishes between human cellular and plasma fibronectin. Using β-galactosidase-fibronectin fusion proteins expressed in E. coli we have demonstrated that this monoclonal antibody is specific for a fibronectin segment (ED) which can be included or omitted from the molecule depending on the pattern of splicing of the mRNA precursors. Furthermore, using the same fusion proteins we have been able to localize precisely the epitopes of two other monoclonal antibodies (IST-1 and IST-2), specific for the heparin-binding domain 5 of fibronectin.

Original languageEnglish
Pages (from-to)269-273
Number of pages5
JournalFEBS Letters
Volume215
Issue number2
DOIs
Publication statusPublished - May 11 1987

Fingerprint

Fibronectins
Escherichia coli
Epitopes
Fusion reactions
Monoclonal Antibodies
Proteins
Galactosidases
RNA Precursors
Heparin
Plasmas
Molecules

Keywords

  • Fibronectin
  • Fusion protein
  • Monoclonal antibody
  • Splicing

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Localization of the cellular-fibronectin-specific epitope recognized by the monoclonal antibody IST-9 using fusion proteins expressed in E. coli. / Carnemolla, Barbara; Borsi, Laura; Zardi, Luciano; Owens, Raymond J.; Baralle, Francisco E.

In: FEBS Letters, Vol. 215, No. 2, 11.05.1987, p. 269-273.

Research output: Contribution to journalArticle

Carnemolla, Barbara ; Borsi, Laura ; Zardi, Luciano ; Owens, Raymond J. ; Baralle, Francisco E. / Localization of the cellular-fibronectin-specific epitope recognized by the monoclonal antibody IST-9 using fusion proteins expressed in E. coli. In: FEBS Letters. 1987 ; Vol. 215, No. 2. pp. 269-273.
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