Abstract
Fragments derived from human plasma fibronectin by enzymatic degradation were tested in the Boyden chamber for chemotactic activity towards various fibroblast strains. The results provide clear evidence that the chemotactic activity is restricted to a defined region of the fibronectin molecule which is the same for various fibroblast strains. The active domain is localized between the collagen binding site and the major heparin binding site, about 170 kDa apart from the N-terminal and about 70 kDa from the C-terminal ends of the two subunit peptide chains.
| Original language | English |
|---|---|
| Pages (from-to) | 222-226 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 156 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jun 13 1983 |
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Keywords
- Boyden chamber
- Fibroblast chemotaxis
- Fibronectin
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Localization of the chemotactic domain in fibronectin. / Albini, A.; Richter, H.; Pontz, B. F.
In: FEBS Letters, Vol. 156, No. 2, 13.06.1983, p. 222-226.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Localization of the chemotactic domain in fibronectin
AU - Albini, A.
AU - Richter, H.
AU - Pontz, B. F.
PY - 1983/6/13
Y1 - 1983/6/13
N2 - Fragments derived from human plasma fibronectin by enzymatic degradation were tested in the Boyden chamber for chemotactic activity towards various fibroblast strains. The results provide clear evidence that the chemotactic activity is restricted to a defined region of the fibronectin molecule which is the same for various fibroblast strains. The active domain is localized between the collagen binding site and the major heparin binding site, about 170 kDa apart from the N-terminal and about 70 kDa from the C-terminal ends of the two subunit peptide chains.
AB - Fragments derived from human plasma fibronectin by enzymatic degradation were tested in the Boyden chamber for chemotactic activity towards various fibroblast strains. The results provide clear evidence that the chemotactic activity is restricted to a defined region of the fibronectin molecule which is the same for various fibroblast strains. The active domain is localized between the collagen binding site and the major heparin binding site, about 170 kDa apart from the N-terminal and about 70 kDa from the C-terminal ends of the two subunit peptide chains.
KW - Boyden chamber
KW - Fibroblast chemotaxis
KW - Fibronectin
UR - http://www.scopus.com/inward/record.url?scp=0020626774&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0020626774&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(83)80500-6
DO - 10.1016/0014-5793(83)80500-6
M3 - Article
C2 - 6852256
AN - SCOPUS:0020626774
VL - 156
SP - 222
EP - 226
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 2
ER -