Localization of the Glutamine Transporter SNAT1 in Rat Cerebral Cortex and Neighboring Structures, with a Note on its Localization in Human Cortex

Marcello Melone, Fiorinta Quagliano, Paolo Barbaresi, Hélène Varoqui, Jeffrey D. Erickson, Fiorenzo Conti

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

SNAT1 mediates glutamine (Gln) influx into neurons and is believed to replenish the transmitters pools of glutamate (Glu) and γ-aminobutyric acid (GABA). We investigated its distribution and cellular localization in the cerebral cortex and neighboring regions of rats and humans using light and electron microscopic immunocytochemical methods with specific antibodies. In the first somatic sensory cortex of rats and in areas 9, 10, 21 and 46 of the human cortex, numerous SNAT1-positive (+) cells were present in the cortical parenchyma and in the white matter; >95% of SNAT1+ cells were neurons, but some were astrocytes. Most SNAT1+ cells were pyramidal neurons, but numerous non-pyramidal neurons were also observed: SNAT1/ GABA double-labeling studies showed that SNAT1 is expressed in all GABA+ neurons. SNAT1/synaptophysin studies showed that

Original languageEnglish
Pages (from-to)562-574
Number of pages13
JournalCerebral Cortex
Volume14
Issue number5
DOIs
Publication statusPublished - May 2004

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Aminobutyrates
Glutamine
Cerebral Cortex
Neurons
Synaptophysin
Pyramidal Cells
Astrocytes
Glutamic Acid
Electrons
Light
Antibodies

Keywords

  • BBB
  • GABA
  • Glutamate
  • Glutamine-glutamate cycle

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Localization of the Glutamine Transporter SNAT1 in Rat Cerebral Cortex and Neighboring Structures, with a Note on its Localization in Human Cortex. / Melone, Marcello; Quagliano, Fiorinta; Barbaresi, Paolo; Varoqui, Hélène; Erickson, Jeffrey D.; Conti, Fiorenzo.

In: Cerebral Cortex, Vol. 14, No. 5, 05.2004, p. 562-574.

Research output: Contribution to journalArticle

Melone, Marcello ; Quagliano, Fiorinta ; Barbaresi, Paolo ; Varoqui, Hélène ; Erickson, Jeffrey D. ; Conti, Fiorenzo. / Localization of the Glutamine Transporter SNAT1 in Rat Cerebral Cortex and Neighboring Structures, with a Note on its Localization in Human Cortex. In: Cerebral Cortex. 2004 ; Vol. 14, No. 5. pp. 562-574.
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