Localization of the laminin α2 chain in normal human skeletal muscle and peripheral nerve: An ultrastructural immunolabeling study

Alessandro Malandrini, Marcello Villanova, Patrizia Sabatelli, Stefane Squarzoni, Jan Six, Paolo Toti, Giancarlo Guazzi, Nadir M. Maraldi

Research output: Contribution to journalArticle

Abstract

A particular form of congenital muscular dystrophy is associated with a deficiency of the tissue-specific basement membrane protein laminin α2. A more precise knowledge of the normal distribution and localization of laminin α2 would be useful in further elucidating the development of this disorder. In this study we used specific electron microscopic techniques, i.e., thin-section fracture labeling and cryoultramicrotomy in combination with immunogold labeling for laminin α2, to determine its ultrastructural localization in normal human muscle and peripheral nerve. Both in muscle and in peripheral nerve, laminin α2 is found to be associated solely with the basal lamina of myofibers and Schwann cells, respectively. Of special interest is the finding that in peripheral nerve, laminin α2 is associated only with myelinated and not with unmyelinated nerve fibers.

Original languageEnglish
Pages (from-to)166-172
Number of pages7
JournalActa Neuropathologica
Volume93
Issue number2
DOIs
Publication statusPublished - 1997

Keywords

  • Basal lamina
  • Immunoelectron microscopy
  • Laminin α2
  • Muscle
  • Schwann cell

ASJC Scopus subject areas

  • Clinical Neurology
  • Pathology and Forensic Medicine
  • Neuroscience(all)

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