Long-distance FRET analysis: A Monte Carlo simulation study

Piotr Bojarski, Leszek Kulak, Katarzyna Walczewska-Szewc, Anna Synak, Vincenzo Manuel Marzullo, Alberto Luini, Sabato D'Auria

Research output: Contribution to journalArticlepeer-review


A new method for extending the utilizable range of Förster resonance energy transfer (FRET) is proposed and tested by the Monte Carlo technique. The obtained results indicate that the efficiency of FRET can be significantly enhanced at a given distance if the energy transfer takes place toward multiple acceptors that are closely located on a macromolecule instead of a single acceptor molecule as it is currently used in FRET analysis. On the other hand, reasonable FRET efficiency can be obtained at significantly longer distances than in the case of a single acceptor. Randomly distributed and parallel orientated acceptor transition moments with respect to the transition moment of the donor molecule have been analyzed as two extreme cases. As expected, a parallel orientation of donor and acceptor transition moments results in a more efficient excitation energy transfer. This finding could be used to directly reveal the assembly/deassembly of large protein complexes in a cell by fluorescence microscopy.

Original languageEnglish
Pages (from-to)10120-10125
Number of pages6
JournalJournal of Physical Chemistry B
Issue number33
Publication statusPublished - Aug 25 2011

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Materials Chemistry
  • Surfaces, Coatings and Films


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