Long-Lasting Anti-Inflammatory and Antinociceptive Effects of Acute Ammonium Glycyrrhizinate Administration: Pharmacological, Biochemical, and Docking Studies

Francesco Maione, Paola Minosi, Amalia Di Giannuario, Federica Raucci, Maria Giovanna Chini, Simona De Vita, Giuseppe Bifulco, Nicola Mascolo, Stefano Pieretti

Research output: Contribution to journalArticlepeer-review

Abstract

The object of the study was to estimate the long-lasting effects induced by ammonium glycyrrhizinate (AG) after a single administration in mice using animal models of pain and inflammation together with biochemical and docking studies. A single intraperitoneal injection of AG was able to produce anti-inflammatory effects in zymosan-induced paw edema and peritonitis. Moreover, in several animal models of pain, such as the writhing test, the formalin test, and hyperalgesia induced by zymosan, AG administered 24 h before the tests was able to induce a strong antinociceptive effect. Molecular docking studies revealed that AG possesses higher affinity for microsomal prostaglandin E synthase type-2 compared to type-1, whereas it seems to locate better in the binding pocket of cyclooxygenase (COX)-2 compared to COX-1. These results demonstrated that AG induced anti-inflammatory and antinociceptive effects until 24-48 h after a single administration thanks to its ability to bind the COX/mPGEs pathway. Taken together, all these findings highlight the potential use of AG for clinical treatment of pain and/or inflammatory-related diseases.

Original languageEnglish
JournalMolecules (Basel, Switzerland)
Volume24
Issue number13
DOIs
Publication statusPublished - Jul 4 2019

Keywords

  • Analgesics/administration & dosage
  • Animals
  • Anti-Inflammatory Agents/administration & dosage
  • Chemokines/metabolism
  • Edema/pathology
  • Formaldehyde
  • Glycyrrhizic Acid/administration & dosage
  • Hyperalgesia/chemically induced
  • Injections, Intraperitoneal
  • Male
  • Mice
  • Molecular Docking Simulation
  • Peritoneal Cavity/pathology
  • Zymosan/administration & dosage

Fingerprint Dive into the research topics of 'Long-Lasting Anti-Inflammatory and Antinociceptive Effects of Acute Ammonium Glycyrrhizinate Administration: Pharmacological, Biochemical, and Docking Studies'. Together they form a unique fingerprint.

Cite this