Long-lasting memory-resting and memory-effector CD4+ T cells in human X-linked agammaglobulinemia

Marino Paroli, Daniele Accapezzato, Vittorio Francavilla, Antonella Insalaco, Alessandro Plebani, Francesco Balsano, Vincenzo Barnaba

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Conflicting results obtained from animal studies suggest that B cells play a role in maintaining long-term T-cell memory and in skewing T-cell response toward a T-helper 2 (TH2) phenotype. X-linked agammaglobulinemia (XLA) is a genetic human disease characterized by the lack of circulating B cells due to the mutation of Bruton tyrosine kinase. This disease thus represents a unique model for studying the role of B lymphocytes in regulating T-cell functions in humans. To this aim, we analyzed hepatitis B envelope antigen (HBenvAg)-specific T-cell memory in a series of XLA patients vaccinated against hepatitis B virus (HBV). We found HBenvAg-specific T lymphocytes producing interferon-γ, interleukin-4, or both in the peripheral blood of XLA patients up to at least 24 months after completing the standard anti-HBV immunization protocol. The HBenvAg-specific T-cell frequencies and the percentage of patients with these responses were not significantly different from healthy vaccinated controls. By combining cell purification and enzyme-linked immunospot assay, we found that effector CD27- T cells, which promptly produced cytokines in response to antigen (Ag), and memory-resting CD27+ T cells, which required Ag restimulation to perform their functions, were maintained in both XLA patients and controls for up to 24 months after the last vaccination boost. These data strongly suggest that B cells are not an absolute requirement for the generation of effective T-cell memory in humans, nor do they seem to influence TH1/TH2 balance.

Original languageEnglish
Pages (from-to)2131-2137
Number of pages7
JournalBlood
Volume99
Issue number6
DOIs
Publication statusPublished - Mar 15 2002

Fingerprint

T-cells
T-Lymphocytes
Data storage equipment
Hepatitis B Antigens
Antigens
B-Lymphocytes
Cells
Viruses
Hepatitis B virus
Immunization
Bruton type agammaglobulinemia
Enzyme-Linked Immunospot Assay
Inborn Genetic Diseases
Lymphocytes
Interleukin-4
Interferons
Purification
Assays
Vaccination
Animals

ASJC Scopus subject areas

  • Hematology

Cite this

Paroli, M., Accapezzato, D., Francavilla, V., Insalaco, A., Plebani, A., Balsano, F., & Barnaba, V. (2002). Long-lasting memory-resting and memory-effector CD4+ T cells in human X-linked agammaglobulinemia. Blood, 99(6), 2131-2137. https://doi.org/10.1182/blood.V99.6.2131

Long-lasting memory-resting and memory-effector CD4+ T cells in human X-linked agammaglobulinemia. / Paroli, Marino; Accapezzato, Daniele; Francavilla, Vittorio; Insalaco, Antonella; Plebani, Alessandro; Balsano, Francesco; Barnaba, Vincenzo.

In: Blood, Vol. 99, No. 6, 15.03.2002, p. 2131-2137.

Research output: Contribution to journalArticle

Paroli, M, Accapezzato, D, Francavilla, V, Insalaco, A, Plebani, A, Balsano, F & Barnaba, V 2002, 'Long-lasting memory-resting and memory-effector CD4+ T cells in human X-linked agammaglobulinemia', Blood, vol. 99, no. 6, pp. 2131-2137. https://doi.org/10.1182/blood.V99.6.2131
Paroli, Marino ; Accapezzato, Daniele ; Francavilla, Vittorio ; Insalaco, Antonella ; Plebani, Alessandro ; Balsano, Francesco ; Barnaba, Vincenzo. / Long-lasting memory-resting and memory-effector CD4+ T cells in human X-linked agammaglobulinemia. In: Blood. 2002 ; Vol. 99, No. 6. pp. 2131-2137.
@article{89c222d619a4419c9fc8119d6d3ffc41,
title = "Long-lasting memory-resting and memory-effector CD4+ T cells in human X-linked agammaglobulinemia",
abstract = "Conflicting results obtained from animal studies suggest that B cells play a role in maintaining long-term T-cell memory and in skewing T-cell response toward a T-helper 2 (TH2) phenotype. X-linked agammaglobulinemia (XLA) is a genetic human disease characterized by the lack of circulating B cells due to the mutation of Bruton tyrosine kinase. This disease thus represents a unique model for studying the role of B lymphocytes in regulating T-cell functions in humans. To this aim, we analyzed hepatitis B envelope antigen (HBenvAg)-specific T-cell memory in a series of XLA patients vaccinated against hepatitis B virus (HBV). We found HBenvAg-specific T lymphocytes producing interferon-γ, interleukin-4, or both in the peripheral blood of XLA patients up to at least 24 months after completing the standard anti-HBV immunization protocol. The HBenvAg-specific T-cell frequencies and the percentage of patients with these responses were not significantly different from healthy vaccinated controls. By combining cell purification and enzyme-linked immunospot assay, we found that effector CD27- T cells, which promptly produced cytokines in response to antigen (Ag), and memory-resting CD27+ T cells, which required Ag restimulation to perform their functions, were maintained in both XLA patients and controls for up to 24 months after the last vaccination boost. These data strongly suggest that B cells are not an absolute requirement for the generation of effective T-cell memory in humans, nor do they seem to influence TH1/TH2 balance.",
author = "Marino Paroli and Daniele Accapezzato and Vittorio Francavilla and Antonella Insalaco and Alessandro Plebani and Francesco Balsano and Vincenzo Barnaba",
year = "2002",
month = "3",
day = "15",
doi = "10.1182/blood.V99.6.2131",
language = "English",
volume = "99",
pages = "2131--2137",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "6",

}

TY - JOUR

T1 - Long-lasting memory-resting and memory-effector CD4+ T cells in human X-linked agammaglobulinemia

AU - Paroli, Marino

AU - Accapezzato, Daniele

AU - Francavilla, Vittorio

AU - Insalaco, Antonella

AU - Plebani, Alessandro

AU - Balsano, Francesco

AU - Barnaba, Vincenzo

PY - 2002/3/15

Y1 - 2002/3/15

N2 - Conflicting results obtained from animal studies suggest that B cells play a role in maintaining long-term T-cell memory and in skewing T-cell response toward a T-helper 2 (TH2) phenotype. X-linked agammaglobulinemia (XLA) is a genetic human disease characterized by the lack of circulating B cells due to the mutation of Bruton tyrosine kinase. This disease thus represents a unique model for studying the role of B lymphocytes in regulating T-cell functions in humans. To this aim, we analyzed hepatitis B envelope antigen (HBenvAg)-specific T-cell memory in a series of XLA patients vaccinated against hepatitis B virus (HBV). We found HBenvAg-specific T lymphocytes producing interferon-γ, interleukin-4, or both in the peripheral blood of XLA patients up to at least 24 months after completing the standard anti-HBV immunization protocol. The HBenvAg-specific T-cell frequencies and the percentage of patients with these responses were not significantly different from healthy vaccinated controls. By combining cell purification and enzyme-linked immunospot assay, we found that effector CD27- T cells, which promptly produced cytokines in response to antigen (Ag), and memory-resting CD27+ T cells, which required Ag restimulation to perform their functions, were maintained in both XLA patients and controls for up to 24 months after the last vaccination boost. These data strongly suggest that B cells are not an absolute requirement for the generation of effective T-cell memory in humans, nor do they seem to influence TH1/TH2 balance.

AB - Conflicting results obtained from animal studies suggest that B cells play a role in maintaining long-term T-cell memory and in skewing T-cell response toward a T-helper 2 (TH2) phenotype. X-linked agammaglobulinemia (XLA) is a genetic human disease characterized by the lack of circulating B cells due to the mutation of Bruton tyrosine kinase. This disease thus represents a unique model for studying the role of B lymphocytes in regulating T-cell functions in humans. To this aim, we analyzed hepatitis B envelope antigen (HBenvAg)-specific T-cell memory in a series of XLA patients vaccinated against hepatitis B virus (HBV). We found HBenvAg-specific T lymphocytes producing interferon-γ, interleukin-4, or both in the peripheral blood of XLA patients up to at least 24 months after completing the standard anti-HBV immunization protocol. The HBenvAg-specific T-cell frequencies and the percentage of patients with these responses were not significantly different from healthy vaccinated controls. By combining cell purification and enzyme-linked immunospot assay, we found that effector CD27- T cells, which promptly produced cytokines in response to antigen (Ag), and memory-resting CD27+ T cells, which required Ag restimulation to perform their functions, were maintained in both XLA patients and controls for up to 24 months after the last vaccination boost. These data strongly suggest that B cells are not an absolute requirement for the generation of effective T-cell memory in humans, nor do they seem to influence TH1/TH2 balance.

UR - http://www.scopus.com/inward/record.url?scp=0037085795&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037085795&partnerID=8YFLogxK

U2 - 10.1182/blood.V99.6.2131

DO - 10.1182/blood.V99.6.2131

M3 - Article

C2 - 11877289

AN - SCOPUS:0037085795

VL - 99

SP - 2131

EP - 2137

JO - Blood

JF - Blood

SN - 0006-4971

IS - 6

ER -