Long noncoding RNA GAS5: A novel marker involved in glucocorticoid response

M. Lucafò, S. De Iudicibus, A. Di Silvestre, M. Pelin, L. Candussio, S. Martelossi, A. Tommasini, E. Piscianz, A. Ventura, G. Decorti

Research output: Contribution to journalArticlepeer-review

Abstract

Glucocorticoids (GCs) exert their effects through regulation of gene expression after activation in the cytoplasm of the glucocorticoid receptor (GR) encoded by NR3C1 gene. A negative feedback mechanism resulting in GR autoregulation has been demonstrated through the binding of the activated receptor to intragenic sequences called GRE-like elements, contained in GR gene.

The long noncoding RNA growth arrest–specific transcript 5 (GAS5) interacts with the activated GR suppressing its transcriptional activity. The aim of this study was to evaluate the possible role of GAS5 and NR3C1 gene expression in the antiproliferative effect of methylprednisolone in peripheral blood mononuclear cells and to correlate the expression with individual sensitivity to GCs. Subjects being poor responders to GCs presented higher levels of GAS5 and NR3C1 in comparison with good responders. We suggest that abnormal levels of GAS5 may alter GC effectiveness, probably interfering with the mechanism of GR autoregulation.

Original languageEnglish
Pages (from-to)94-99
Number of pages6
JournalCurrent Molecular Medicine
Volume15
Issue number1
Publication statusPublished - Jan 1 2015

Keywords

  • GAS5
  • Gene expression
  • Glucocorticoid receptor
  • Long noncoding RNA
  • Methylprednisolone
  • NR3C1 gene
  • Proliferation assay

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Molecular Medicine
  • Medicine(all)

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