TY - JOUR
T1 - Long term bed rest with and without vibration exercise countermeasures
T2 - Effects on human muscle protein dysregulation
AU - Moriggi, Manuela
AU - Vasso, Michele
AU - Fania, Chiara
AU - Capitanio, Daniele
AU - Bonifacio, Gaetano
AU - Salanova, Michele
AU - Blottner, Dieter
AU - Rittweger, Jörn
AU - Felsenberg, Dieter
AU - Cerretelli, Paolo
AU - Gelfi, Cecilia
PY - 2010/11
Y1 - 2010/11
N2 - The present investigation, the first in the field, was aimed at analyzing differentially, on individual samples, the effects of 55 days of horizontal bed rest, a model for microgravity, on myosin heavy and myosin light chain isoforms distribution (by SDS) and on the proteome (by 2-D DIGE and MS) in the vastus lateralis (VL), a mixed type II/I (~50:50%) head of the quadriceps and in the calf soleus (SOL), a predominantly slow (~35:65%) twitch muscle. Two separate studies were performed on six subjects without (BR) and six with resistive vibration exercise (RVE) countermeasures, respectively. Both VL and SOL underwent in BR decrements of ~15% in cross-sectional area and of ~22% in maximal torque that were prevented by RVE. Myosin heavy chain distribution showed increased type I and decreased type IIA in BR both in VL and in SOL, the opposite with RVE. A substantial downregulation of proteins involved in aerobic metabolism characterized both in SOL and VL in BR. RVE reversed the pattern more in VL than in SOL, whereas proteins involved in anaerobic glycolysis were upregulated. Proteins from the Z-disk region and from costamers were differently dysregulated during bed rest (both BR and RVE), particularly in VL.
AB - The present investigation, the first in the field, was aimed at analyzing differentially, on individual samples, the effects of 55 days of horizontal bed rest, a model for microgravity, on myosin heavy and myosin light chain isoforms distribution (by SDS) and on the proteome (by 2-D DIGE and MS) in the vastus lateralis (VL), a mixed type II/I (~50:50%) head of the quadriceps and in the calf soleus (SOL), a predominantly slow (~35:65%) twitch muscle. Two separate studies were performed on six subjects without (BR) and six with resistive vibration exercise (RVE) countermeasures, respectively. Both VL and SOL underwent in BR decrements of ~15% in cross-sectional area and of ~22% in maximal torque that were prevented by RVE. Myosin heavy chain distribution showed increased type I and decreased type IIA in BR both in VL and in SOL, the opposite with RVE. A substantial downregulation of proteins involved in aerobic metabolism characterized both in SOL and VL in BR. RVE reversed the pattern more in VL than in SOL, whereas proteins involved in anaerobic glycolysis were upregulated. Proteins from the Z-disk region and from costamers were differently dysregulated during bed rest (both BR and RVE), particularly in VL.
KW - 2-D DIGE
KW - Bed rest
KW - Biomedicine
KW - Exercise countermeasures
KW - Mass Spectrometry
KW - Muscle
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U2 - 10.1002/pmic.200900817
DO - 10.1002/pmic.200900817
M3 - Article
C2 - 20957755
AN - SCOPUS:78049354620
VL - 10
SP - 3756
EP - 3774
JO - Proteomics
JF - Proteomics
SN - 1615-9853
IS - 21
ER -