Long term bed rest with and without vibration exercise countermeasures: Effects on human muscle protein dysregulation

Manuela Moriggi, Michele Vasso, Chiara Fania, Daniele Capitanio, Gaetano Bonifacio, Michele Salanova, Dieter Blottner, Jörn Rittweger, Dieter Felsenberg, Paolo Cerretelli, Cecilia Gelfi

Research output: Contribution to journalArticlepeer-review


The present investigation, the first in the field, was aimed at analyzing differentially, on individual samples, the effects of 55 days of horizontal bed rest, a model for microgravity, on myosin heavy and myosin light chain isoforms distribution (by SDS) and on the proteome (by 2-D DIGE and MS) in the vastus lateralis (VL), a mixed type II/I (~50:50%) head of the quadriceps and in the calf soleus (SOL), a predominantly slow (~35:65%) twitch muscle. Two separate studies were performed on six subjects without (BR) and six with resistive vibration exercise (RVE) countermeasures, respectively. Both VL and SOL underwent in BR decrements of ~15% in cross-sectional area and of ~22% in maximal torque that were prevented by RVE. Myosin heavy chain distribution showed increased type I and decreased type IIA in BR both in VL and in SOL, the opposite with RVE. A substantial downregulation of proteins involved in aerobic metabolism characterized both in SOL and VL in BR. RVE reversed the pattern more in VL than in SOL, whereas proteins involved in anaerobic glycolysis were upregulated. Proteins from the Z-disk region and from costamers were differently dysregulated during bed rest (both BR and RVE), particularly in VL.

Original languageEnglish
Pages (from-to)3756-3774
Number of pages19
Issue number21
Publication statusPublished - Nov 2010


  • 2-D DIGE
  • Bed rest
  • Biomedicine
  • Exercise countermeasures
  • Mass Spectrometry
  • Muscle

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry


Dive into the research topics of 'Long term bed rest with and without vibration exercise countermeasures: Effects on human muscle protein dysregulation'. Together they form a unique fingerprint.

Cite this