Long term breeding of the Lmna G609G progeric mouse: Characterization of homozygous and heterozygous models

Anna Zaghini, Giuseppe Sarli, Catia Barboni, Mara Sanapo, Valeria Pellegrino, Alessia Diana, Nikolina Linta, Julie Rambaldi, Maria Rosaria D'Apice, Michela Murdocca, Massimiliano Baleani, Fabio Baruffaldi, Roberta Fognani, Rosaria Mecca, Anna Festa, Serenella Papparella, Orlando Paciello, Francesco Prisco, Cristina Capanni, Manuela LoiElisa Schena, Giovanna Lattanzi, Stefano Squarzoni

Research output: Contribution to journalArticlepeer-review

Abstract

The transgenic LmnaG609G progeric mouse represents an outstanding animal model for studying the human Hutchinson-Gilford Progeria Syndrome (HGPS) caused by a mutation in the LMNA gene, coding for the nuclear envelope protein Lamin A/C, and, as an important, more general scope, for studying the complex process governing physiological aging in humans. Here we give a comprehensive description of the peculiarities related to the breeding of LmnaG609G mice over a prolonged period of time, and of many features observed in a large colony for a 2-years period. We describe the breeding and housing conditions underlining the possible interference of the genetic background on the phenotype expression. This information represents a useful tool when planning and interpreting studies on the LmnaG609G mouse model, complementing any specific data already reported in the literature about this model since its production. It is also particularly relevant for the heterozygous mouse, which mirrors the genotype of the human pathology however requires an extended time to manifest symptoms and to be carefully studied.

Original languageEnglish
Pages (from-to)1-18
Number of pages18
JournalExperimental Gerontology
Volume130
DOIs
Publication statusE-pub ahead of print - Nov 30 2019

Keywords

  • Aging
  • Animal model breeding
  • Bone strength
  • Hutchinson-Gilford Progeria Syndrome (HGPS)
  • Kyphosis
  • Quality of life

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