Long-term changes to in vitro preserved bioengineered human trachea and their implications for decellularized tissues

Silvia Baiguera, Costantino Del Gaudio, Massimo O. Jaus, Leonardo Polizzi, Alessandro Gonfiotti, Camilla E. Comin, Alessandra Bianco, Domenico Ribatti, Doris A. Taylor, Paolo Macchiarini

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Bioengineered tissues created for transplant will be expected to survive and contribute to function over the lifetime of the individual. To evaluate potential intrinsic changes and degradation of the extracellular matrix of decellularized human tissue scaffolds, human decellularized tracheas were evaluated over a one year period in vitro. Human tracheas were decellularized and stored for one year in phosphate-buffered saline at 4 °C in the presence of antibiotics and anti-mycotics, and their structural, mechanical, and angiogenic properties compared to baseline values. Results showed that stored human decellularized tracheas were increasingly degraded resulting in a loss of extracellular matrix architecture - in particular of collagenous and elastic fiber structure -and decreased mechanical and angiogenic properties. The mechanical alterations of the extracellular matrix but not the deterioration and microstructure were not improved by using a natural cross-linking agent. These findings demonstrate that human decellularized tracheas, stored for one year in phosphate-buffered saline solution at 4 °C, would not meet the demands for a tissue engineering matrix and likely would not yield a suitable graft for lifelong implantation. The degradation phenomenon observed in vitro may be further enhanced in vivo, having clinical relevance for tissues that will be transplanted long-term and this should be carefully evaluated in pre-clinical settings.

Original languageEnglish
Pages (from-to)3662-3672
Number of pages11
JournalBiomaterials
Volume33
Issue number14
DOIs
Publication statusPublished - May 2012

Fingerprint

Trachea
Tissue
Extracellular Matrix
Phosphates
Tissue Scaffolds
Degradation
Transplants
Antibiotics
Tissue engineering
Sodium Chloride
Grafts
Ion implantation
Elastic Tissue
Deterioration
Tissue Engineering
Anti-Bacterial Agents
Microstructure
In Vitro Techniques
Fibers

Keywords

  • Angiogenesis
  • Cross-linking
  • Decellularized human trachea
  • Degradation
  • Long-term integrity
  • Mechanical properties

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Baiguera, S., Del Gaudio, C., Jaus, M. O., Polizzi, L., Gonfiotti, A., Comin, C. E., ... Macchiarini, P. (2012). Long-term changes to in vitro preserved bioengineered human trachea and their implications for decellularized tissues. Biomaterials, 33(14), 3662-3672. https://doi.org/10.1016/j.biomaterials.2012.01.064

Long-term changes to in vitro preserved bioengineered human trachea and their implications for decellularized tissues. / Baiguera, Silvia; Del Gaudio, Costantino; Jaus, Massimo O.; Polizzi, Leonardo; Gonfiotti, Alessandro; Comin, Camilla E.; Bianco, Alessandra; Ribatti, Domenico; Taylor, Doris A.; Macchiarini, Paolo.

In: Biomaterials, Vol. 33, No. 14, 05.2012, p. 3662-3672.

Research output: Contribution to journalArticle

Baiguera, S, Del Gaudio, C, Jaus, MO, Polizzi, L, Gonfiotti, A, Comin, CE, Bianco, A, Ribatti, D, Taylor, DA & Macchiarini, P 2012, 'Long-term changes to in vitro preserved bioengineered human trachea and their implications for decellularized tissues', Biomaterials, vol. 33, no. 14, pp. 3662-3672. https://doi.org/10.1016/j.biomaterials.2012.01.064
Baiguera, Silvia ; Del Gaudio, Costantino ; Jaus, Massimo O. ; Polizzi, Leonardo ; Gonfiotti, Alessandro ; Comin, Camilla E. ; Bianco, Alessandra ; Ribatti, Domenico ; Taylor, Doris A. ; Macchiarini, Paolo. / Long-term changes to in vitro preserved bioengineered human trachea and their implications for decellularized tissues. In: Biomaterials. 2012 ; Vol. 33, No. 14. pp. 3662-3672.
@article{3370b90a6f6348d7ba53ebddc72d9d9d,
title = "Long-term changes to in vitro preserved bioengineered human trachea and their implications for decellularized tissues",
abstract = "Bioengineered tissues created for transplant will be expected to survive and contribute to function over the lifetime of the individual. To evaluate potential intrinsic changes and degradation of the extracellular matrix of decellularized human tissue scaffolds, human decellularized tracheas were evaluated over a one year period in vitro. Human tracheas were decellularized and stored for one year in phosphate-buffered saline at 4 °C in the presence of antibiotics and anti-mycotics, and their structural, mechanical, and angiogenic properties compared to baseline values. Results showed that stored human decellularized tracheas were increasingly degraded resulting in a loss of extracellular matrix architecture - in particular of collagenous and elastic fiber structure -and decreased mechanical and angiogenic properties. The mechanical alterations of the extracellular matrix but not the deterioration and microstructure were not improved by using a natural cross-linking agent. These findings demonstrate that human decellularized tracheas, stored for one year in phosphate-buffered saline solution at 4 °C, would not meet the demands for a tissue engineering matrix and likely would not yield a suitable graft for lifelong implantation. The degradation phenomenon observed in vitro may be further enhanced in vivo, having clinical relevance for tissues that will be transplanted long-term and this should be carefully evaluated in pre-clinical settings.",
keywords = "Angiogenesis, Cross-linking, Decellularized human trachea, Degradation, Long-term integrity, Mechanical properties",
author = "Silvia Baiguera and {Del Gaudio}, Costantino and Jaus, {Massimo O.} and Leonardo Polizzi and Alessandro Gonfiotti and Comin, {Camilla E.} and Alessandra Bianco and Domenico Ribatti and Taylor, {Doris A.} and Paolo Macchiarini",
year = "2012",
month = "5",
doi = "10.1016/j.biomaterials.2012.01.064",
language = "English",
volume = "33",
pages = "3662--3672",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "14",

}

TY - JOUR

T1 - Long-term changes to in vitro preserved bioengineered human trachea and their implications for decellularized tissues

AU - Baiguera, Silvia

AU - Del Gaudio, Costantino

AU - Jaus, Massimo O.

AU - Polizzi, Leonardo

AU - Gonfiotti, Alessandro

AU - Comin, Camilla E.

AU - Bianco, Alessandra

AU - Ribatti, Domenico

AU - Taylor, Doris A.

AU - Macchiarini, Paolo

PY - 2012/5

Y1 - 2012/5

N2 - Bioengineered tissues created for transplant will be expected to survive and contribute to function over the lifetime of the individual. To evaluate potential intrinsic changes and degradation of the extracellular matrix of decellularized human tissue scaffolds, human decellularized tracheas were evaluated over a one year period in vitro. Human tracheas were decellularized and stored for one year in phosphate-buffered saline at 4 °C in the presence of antibiotics and anti-mycotics, and their structural, mechanical, and angiogenic properties compared to baseline values. Results showed that stored human decellularized tracheas were increasingly degraded resulting in a loss of extracellular matrix architecture - in particular of collagenous and elastic fiber structure -and decreased mechanical and angiogenic properties. The mechanical alterations of the extracellular matrix but not the deterioration and microstructure were not improved by using a natural cross-linking agent. These findings demonstrate that human decellularized tracheas, stored for one year in phosphate-buffered saline solution at 4 °C, would not meet the demands for a tissue engineering matrix and likely would not yield a suitable graft for lifelong implantation. The degradation phenomenon observed in vitro may be further enhanced in vivo, having clinical relevance for tissues that will be transplanted long-term and this should be carefully evaluated in pre-clinical settings.

AB - Bioengineered tissues created for transplant will be expected to survive and contribute to function over the lifetime of the individual. To evaluate potential intrinsic changes and degradation of the extracellular matrix of decellularized human tissue scaffolds, human decellularized tracheas were evaluated over a one year period in vitro. Human tracheas were decellularized and stored for one year in phosphate-buffered saline at 4 °C in the presence of antibiotics and anti-mycotics, and their structural, mechanical, and angiogenic properties compared to baseline values. Results showed that stored human decellularized tracheas were increasingly degraded resulting in a loss of extracellular matrix architecture - in particular of collagenous and elastic fiber structure -and decreased mechanical and angiogenic properties. The mechanical alterations of the extracellular matrix but not the deterioration and microstructure were not improved by using a natural cross-linking agent. These findings demonstrate that human decellularized tracheas, stored for one year in phosphate-buffered saline solution at 4 °C, would not meet the demands for a tissue engineering matrix and likely would not yield a suitable graft for lifelong implantation. The degradation phenomenon observed in vitro may be further enhanced in vivo, having clinical relevance for tissues that will be transplanted long-term and this should be carefully evaluated in pre-clinical settings.

KW - Angiogenesis

KW - Cross-linking

KW - Decellularized human trachea

KW - Degradation

KW - Long-term integrity

KW - Mechanical properties

UR - http://www.scopus.com/inward/record.url?scp=84857786353&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84857786353&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2012.01.064

DO - 10.1016/j.biomaterials.2012.01.064

M3 - Article

C2 - 22349289

AN - SCOPUS:84857786353

VL - 33

SP - 3662

EP - 3672

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 14

ER -