Long term cryopreservation in 5% DMSO maintains unchanged CD34+ cells viability and allows satisfactory hematological engraftment after peripheral blood stem cell transplantation

L. Abbruzzese, F. Agostini, C. Durante, R. T. Toffola, M. Rupolo, F. M. Rossi, A. Lleshi, S. Zanolin, M. Michieli, M. Mazzucato

Research output: Contribution to journalArticle


Peripheral blood stem cell cryopreservation is associated with cell damage and decreased viability. We evaluated the impact of up to 10 years of cryopreservation (5% DMSO) on viability of CD34+ cells utilizing graft samples of consecutive patients (2002-2012) with different malignancies who underwent stem cell collection and transplantation. Viability of CD34+ cells from oncohaematological patients measured after 5 weeks (97·2 ± 0·6%) or after 9-10 years of cryopreservation (95·9 ± 0·5%) was unaffected. Haemoglobin, granulocyte and platelet recovery after transplantation of long-term cryopreserved grafts occurred within 8-13 days. CD34+ stem cells can be safely stored up to 9-10 years, without affecting cell viability and clinical effectiveness.

Original languageEnglish
Pages (from-to)77-80
Number of pages4
JournalVox Sanguinis
Issue number1
Publication statusPublished - Jul 2013



  • 5% DMSO
  • CD34 viability
  • Engraftment
  • EVA-teflon double bags
  • Long-term cryopreservation

ASJC Scopus subject areas

  • Hematology

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