Long-term haemopoiesis in human fetal liver cell cultures

M. D. Cappellini, C. G. Potter, W. G. Wood

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Haemopoiesis in human fetal liver is almost entirely restricted to the erythroid series but when fetal liver cells were cultured under conditions established for the long-term maintenance of adult marrow haemopoiesis, a rapid switch to granulopoiesis was observed. Erythroid progenitor cells (BFU-E) rapidly disappeared, even though no humoral or cellular inhibitors of erythropoiesis could be detected, while myeloid progenitors (CFU-GM) increased in number. When the fetal liver cells were seeded onto stromal layers derived from adult marrow, in which endogenous haemopoiesis had ceased, granulopoiesis was established and maintained for more than a year, considerably longer than has previously been achieved with human haemopoietic cells.

Original languageEnglish
Pages (from-to)61-70
Number of pages10
JournalBritish Journal of Haematology
Volume57
Issue number1
Publication statusPublished - 1984

Fingerprint

Erythroid Precursor Cells
Cell Culture Techniques
Liver
Bone Marrow
Granulocyte-Macrophage Progenitor Cells
Erythropoiesis
Cultured Cells

ASJC Scopus subject areas

  • Hematology

Cite this

Long-term haemopoiesis in human fetal liver cell cultures. / Cappellini, M. D.; Potter, C. G.; Wood, W. G.

In: British Journal of Haematology, Vol. 57, No. 1, 1984, p. 61-70.

Research output: Contribution to journalArticle

Cappellini, M. D. ; Potter, C. G. ; Wood, W. G. / Long-term haemopoiesis in human fetal liver cell cultures. In: British Journal of Haematology. 1984 ; Vol. 57, No. 1. pp. 61-70.
@article{28b4c077dd2b4d958b116bfae6eafb4f,
title = "Long-term haemopoiesis in human fetal liver cell cultures",
abstract = "Haemopoiesis in human fetal liver is almost entirely restricted to the erythroid series but when fetal liver cells were cultured under conditions established for the long-term maintenance of adult marrow haemopoiesis, a rapid switch to granulopoiesis was observed. Erythroid progenitor cells (BFU-E) rapidly disappeared, even though no humoral or cellular inhibitors of erythropoiesis could be detected, while myeloid progenitors (CFU-GM) increased in number. When the fetal liver cells were seeded onto stromal layers derived from adult marrow, in which endogenous haemopoiesis had ceased, granulopoiesis was established and maintained for more than a year, considerably longer than has previously been achieved with human haemopoietic cells.",
author = "Cappellini, {M. D.} and Potter, {C. G.} and Wood, {W. G.}",
year = "1984",
language = "English",
volume = "57",
pages = "61--70",
journal = "British Journal of Haematology",
issn = "0007-1048",
publisher = "John Wiley & Sons, Ltd (10.1111)",
number = "1",

}

TY - JOUR

T1 - Long-term haemopoiesis in human fetal liver cell cultures

AU - Cappellini, M. D.

AU - Potter, C. G.

AU - Wood, W. G.

PY - 1984

Y1 - 1984

N2 - Haemopoiesis in human fetal liver is almost entirely restricted to the erythroid series but when fetal liver cells were cultured under conditions established for the long-term maintenance of adult marrow haemopoiesis, a rapid switch to granulopoiesis was observed. Erythroid progenitor cells (BFU-E) rapidly disappeared, even though no humoral or cellular inhibitors of erythropoiesis could be detected, while myeloid progenitors (CFU-GM) increased in number. When the fetal liver cells were seeded onto stromal layers derived from adult marrow, in which endogenous haemopoiesis had ceased, granulopoiesis was established and maintained for more than a year, considerably longer than has previously been achieved with human haemopoietic cells.

AB - Haemopoiesis in human fetal liver is almost entirely restricted to the erythroid series but when fetal liver cells were cultured under conditions established for the long-term maintenance of adult marrow haemopoiesis, a rapid switch to granulopoiesis was observed. Erythroid progenitor cells (BFU-E) rapidly disappeared, even though no humoral or cellular inhibitors of erythropoiesis could be detected, while myeloid progenitors (CFU-GM) increased in number. When the fetal liver cells were seeded onto stromal layers derived from adult marrow, in which endogenous haemopoiesis had ceased, granulopoiesis was established and maintained for more than a year, considerably longer than has previously been achieved with human haemopoietic cells.

UR - http://www.scopus.com/inward/record.url?scp=0021261701&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021261701&partnerID=8YFLogxK

M3 - Article

VL - 57

SP - 61

EP - 70

JO - British Journal of Haematology

JF - British Journal of Haematology

SN - 0007-1048

IS - 1

ER -