Long-term hematologic reconstitution after autologous peripheral blood progenitor cell transplantation: A comparison between controlled-rate freezing and uncontrolled-rate freezing at 80°C

Mauro Montanari, Debora Capelli, Antonella Poloni, Danilo Massidda, Marino Brunori, Luca Spitaleri, Massimo Offidani, Moira Lucesole, Maria C. Masia, Florinda Balducci, Cristina Refe, Mario Piani, Pietro Leoni, Attilio Olivieri

Research output: Contribution to journalArticle

Abstract

BACKGROUND: The most widely used system for peripheral blood progenitor cell (PBPC) cryopreservation is controlled-rate freezing (CRF). Uncontrolled-rate freezing (URF) at -80°C has also been used, but its clinical impact has not been studied sufficiently yet. STUDY DESIGN AND METHODS: Two groups of patients were compared: Group A consisted of 69 patients autotransplanted with PBPCs cryopreserved with CRF; Group B consisted of 192 patients autotransplanted with PBPCs cryopreserved with URF at -80°C. The same cryoprotectant solution and storage system were used. RESULTS: A significant delay of hematologic reconstitution (HR) in the URF group was observed for neutrophils greater than 0.5 × 109 per L and for platelets greater than 20 × 109 per L and greater than 50 × 109 per L; we did not observe any differences in the clinical course. The long-term HR was comparable in the two groups, all patients showed stable engraftment, and no late graft failures were observed. CONCLUSION: Our study confirms that URF is safe and allows sustained long-term engraftment without increasing the risks of transplantation, even though the early engraftment after URF is slower.

Original languageEnglish
Pages (from-to)42-49
Number of pages8
JournalTransfusion
Volume43
Issue number1
DOIs
Publication statusPublished - Jan 1 2003

ASJC Scopus subject areas

  • Hematology
  • Immunology

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    Montanari, M., Capelli, D., Poloni, A., Massidda, D., Brunori, M., Spitaleri, L., Offidani, M., Lucesole, M., Masia, M. C., Balducci, F., Refe, C., Piani, M., Leoni, P., & Olivieri, A. (2003). Long-term hematologic reconstitution after autologous peripheral blood progenitor cell transplantation: A comparison between controlled-rate freezing and uncontrolled-rate freezing at 80°C. Transfusion, 43(1), 42-49. https://doi.org/10.1046/j.1537-2995.2003.00271.x