TY - JOUR
T1 - Long-term hematologic reconstitution after autologous peripheral blood progenitor cell transplantation
T2 - A comparison between controlled-rate freezing and uncontrolled-rate freezing at 80°C
AU - Montanari, Mauro
AU - Capelli, Debora
AU - Poloni, Antonella
AU - Massidda, Danilo
AU - Brunori, Marino
AU - Spitaleri, Luca
AU - Offidani, Massimo
AU - Lucesole, Moira
AU - Masia, Maria C.
AU - Balducci, Florinda
AU - Refe, Cristina
AU - Piani, Mario
AU - Leoni, Pietro
AU - Olivieri, Attilio
PY - 2003/1/1
Y1 - 2003/1/1
N2 - BACKGROUND: The most widely used system for peripheral blood progenitor cell (PBPC) cryopreservation is controlled-rate freezing (CRF). Uncontrolled-rate freezing (URF) at -80°C has also been used, but its clinical impact has not been studied sufficiently yet. STUDY DESIGN AND METHODS: Two groups of patients were compared: Group A consisted of 69 patients autotransplanted with PBPCs cryopreserved with CRF; Group B consisted of 192 patients autotransplanted with PBPCs cryopreserved with URF at -80°C. The same cryoprotectant solution and storage system were used. RESULTS: A significant delay of hematologic reconstitution (HR) in the URF group was observed for neutrophils greater than 0.5 × 109 per L and for platelets greater than 20 × 109 per L and greater than 50 × 109 per L; we did not observe any differences in the clinical course. The long-term HR was comparable in the two groups, all patients showed stable engraftment, and no late graft failures were observed. CONCLUSION: Our study confirms that URF is safe and allows sustained long-term engraftment without increasing the risks of transplantation, even though the early engraftment after URF is slower.
AB - BACKGROUND: The most widely used system for peripheral blood progenitor cell (PBPC) cryopreservation is controlled-rate freezing (CRF). Uncontrolled-rate freezing (URF) at -80°C has also been used, but its clinical impact has not been studied sufficiently yet. STUDY DESIGN AND METHODS: Two groups of patients were compared: Group A consisted of 69 patients autotransplanted with PBPCs cryopreserved with CRF; Group B consisted of 192 patients autotransplanted with PBPCs cryopreserved with URF at -80°C. The same cryoprotectant solution and storage system were used. RESULTS: A significant delay of hematologic reconstitution (HR) in the URF group was observed for neutrophils greater than 0.5 × 109 per L and for platelets greater than 20 × 109 per L and greater than 50 × 109 per L; we did not observe any differences in the clinical course. The long-term HR was comparable in the two groups, all patients showed stable engraftment, and no late graft failures were observed. CONCLUSION: Our study confirms that URF is safe and allows sustained long-term engraftment without increasing the risks of transplantation, even though the early engraftment after URF is slower.
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U2 - 10.1046/j.1537-2995.2003.00271.x
DO - 10.1046/j.1537-2995.2003.00271.x
M3 - Article
C2 - 12519429
AN - SCOPUS:0037224916
VL - 43
SP - 42
EP - 49
JO - Transfusion
JF - Transfusion
SN - 0041-1132
IS - 1
ER -