Long-term human B cell lines dependent on interleukin-4 and antibody to CD40

Jacques Banchereau; Paolo De Paoli; Alain Vallé; Eric Garcia; Françoise Rousset

Long-term human B cell lines dependent on interleukin-4 and antibody to CD40

Jacques Banchereau, Paolo De Paoli, Alain Vallé, Eric Garcia, Françoise Rousset

Centro di Riferimento Oncologico

Research output: Contribution to journal › Article › peer-review

Abstract

CD40 is a 45- to 50-kilodalton transmembrane glycoprotein expressed on B lymphocytes, epithelial cells, and some carcinoma cell lines. Human resting B lymphocytes entered a state of sustained proliferation when incubated with both the mouse fibroblastic Ltk^- cell line that had been transfected with the human Fc receptor (FcγRII/CDw32) and monoclonal antibodies to CD40. In combination with interleukin-4, factor-dependent long-term normal human B cell lines were generated that were consistently negative for Epstein-Barr viral infection. Thus, cross-linking of CD40 is likely to represent an important phenomenon in the clonal expansion of B cells.

Original language	English
Pages (from-to)	70-72
Number of pages	3
Journal	Science
Volume	251
Issue number	4989
Publication status	Published - Jan 4 1991

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title = "Long-term human B cell lines dependent on interleukin-4 and antibody to CD40",

abstract = "CD40 is a 45- to 50-kilodalton transmembrane glycoprotein expressed on B lymphocytes, epithelial cells, and some carcinoma cell lines. Human resting B lymphocytes entered a state of sustained proliferation when incubated with both the mouse fibroblastic Ltk- cell line that had been transfected with the human Fc receptor (FcγRII/CDw32) and monoclonal antibodies to CD40. In combination with interleukin-4, factor-dependent long-term normal human B cell lines were generated that were consistently negative for Epstein-Barr viral infection. Thus, cross-linking of CD40 is likely to represent an important phenomenon in the clonal expansion of B cells.",

author = "Jacques Banchereau and {De Paoli}, Paolo and Alain Vall{\'e} and Eric Garcia and Fran{\c c}oise Rousset",

year = "1991",

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language = "English",

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T1 - Long-term human B cell lines dependent on interleukin-4 and antibody to CD40

AU - Banchereau, Jacques

AU - De Paoli, Paolo

AU - Vallé, Alain

AU - Garcia, Eric

AU - Rousset, Françoise

PY - 1991/1/4

Y1 - 1991/1/4

N2 - CD40 is a 45- to 50-kilodalton transmembrane glycoprotein expressed on B lymphocytes, epithelial cells, and some carcinoma cell lines. Human resting B lymphocytes entered a state of sustained proliferation when incubated with both the mouse fibroblastic Ltk- cell line that had been transfected with the human Fc receptor (FcγRII/CDw32) and monoclonal antibodies to CD40. In combination with interleukin-4, factor-dependent long-term normal human B cell lines were generated that were consistently negative for Epstein-Barr viral infection. Thus, cross-linking of CD40 is likely to represent an important phenomenon in the clonal expansion of B cells.

AB - CD40 is a 45- to 50-kilodalton transmembrane glycoprotein expressed on B lymphocytes, epithelial cells, and some carcinoma cell lines. Human resting B lymphocytes entered a state of sustained proliferation when incubated with both the mouse fibroblastic Ltk- cell line that had been transfected with the human Fc receptor (FcγRII/CDw32) and monoclonal antibodies to CD40. In combination with interleukin-4, factor-dependent long-term normal human B cell lines were generated that were consistently negative for Epstein-Barr viral infection. Thus, cross-linking of CD40 is likely to represent an important phenomenon in the clonal expansion of B cells.

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JO - Science

JF - Science

SN - 0036-8075

IS - 4989

ER -

Long-term human B cell lines dependent on interleukin-4 and antibody to CD40

Abstract

ASJC Scopus subject areas

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