Long-term human B cell lines dependent on interleukin-4 and antibody to CD40

Jacques Banchereau, Paolo De Paoli, Alain Vallé, Eric Garcia, Françoise Rousset

Research output: Contribution to journalArticlepeer-review

Abstract

CD40 is a 45- to 50-kilodalton transmembrane glycoprotein expressed on B lymphocytes, epithelial cells, and some carcinoma cell lines. Human resting B lymphocytes entered a state of sustained proliferation when incubated with both the mouse fibroblastic Ltk- cell line that had been transfected with the human Fc receptor (FcγRII/CDw32) and monoclonal antibodies to CD40. In combination with interleukin-4, factor-dependent long-term normal human B cell lines were generated that were consistently negative for Epstein-Barr viral infection. Thus, cross-linking of CD40 is likely to represent an important phenomenon in the clonal expansion of B cells.

Original languageEnglish
Pages (from-to)70-72
Number of pages3
JournalScience
Volume251
Issue number4989
Publication statusPublished - Jan 4 1991

ASJC Scopus subject areas

  • General

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