Long-term vitamin E supplementation fails to reduce lipid peroxidation in people at cardiovascular risk: Analysis of underlying factors

Chiara Chiabrando, Fausto Avanzini, Claudia Rivalta, Fabio Colombo, Roberto Fanelli, Gaetana Palumbo, Maria Carla Roncaglioni, Marina Bosisio Pioltelli, Alberto Capra, Mario Cristofari, Susanna Rossi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: Antioxidant supplementation with vitamin E had no effect in the prevention of cardiovascular diseases (CVD) in three recent large, randomized clinical trials. In order to reassess critically the role of vitamin E in CVD prevention, it is important to establish whether these results are related to a lack of antioxidant action. Methods: We examined the in vivo antioxidant effect of vitamin E (300 mg/day for about three years) in 144 participants in the Primary Prevention Project (females and males, aged ≥ 50 y, with at least one major CV risk factor, but no history of CVD). Urinary 8-epi-PGF (isoprostane F-III or 15-F2t-isoP), a validated biomarker of lipid peroxidation, was measured by mass spectrometry. Results: Urinary excretion of 8-epi-PGF [pg/mg creatinine, median (range)] was 141 (67-498) in treated and 148 (76-561) in untreated subjects (p = 0.10). Taking into account possible confounding variables, multiple regression analysis confirmed that vitamin E had no significant effect on this biomarker. Levels of 8-epi-PGF were in the normal range for most subjects, except smokers and those with uncontrolled blood pressure or hyperglycemia. Conclusions: Prolonged vitamin E supplementation did not reduce lipid peroxidation in subjects with major cardiovascular risk factors. The observation that the rate of lipid peroxidation was near normal in a large proportion of subjects may help explain why vitamin E was not effective as an antioxidant in the PPP study and was ineffective for CVD prevention in large scale trials.

Original languageEnglish
Article number5
JournalCurrent Controlled Trials in Cardiovascular Medicine
Volume3
Publication statusPublished - Mar 19 2002

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Vitamin E
Lipid Peroxidation
Statistical Factor Analysis
Dinoprost
Cardiovascular Diseases
Antioxidants
Biomarkers
F2-Isoprostanes
Confounding Factors (Epidemiology)
Primary Prevention
Hyperglycemia
Mass Spectrometry
Creatinine
Reference Values
Randomized Controlled Trials
Regression Analysis
Blood Pressure

Keywords

  • Cardiovascular prevention
  • F2-isoprostane
  • Hypertension
  • Lipid peroxidation
  • Vitamin E

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Long-term vitamin E supplementation fails to reduce lipid peroxidation in people at cardiovascular risk : Analysis of underlying factors. / Chiabrando, Chiara; Avanzini, Fausto; Rivalta, Claudia; Colombo, Fabio; Fanelli, Roberto; Palumbo, Gaetana; Roncaglioni, Maria Carla; Pioltelli, Marina Bosisio; Capra, Alberto; Cristofari, Mario; Rossi, Susanna.

In: Current Controlled Trials in Cardiovascular Medicine, Vol. 3, 5, 19.03.2002.

Research output: Contribution to journalArticle

Chiabrando, Chiara ; Avanzini, Fausto ; Rivalta, Claudia ; Colombo, Fabio ; Fanelli, Roberto ; Palumbo, Gaetana ; Roncaglioni, Maria Carla ; Pioltelli, Marina Bosisio ; Capra, Alberto ; Cristofari, Mario ; Rossi, Susanna. / Long-term vitamin E supplementation fails to reduce lipid peroxidation in people at cardiovascular risk : Analysis of underlying factors. In: Current Controlled Trials in Cardiovascular Medicine. 2002 ; Vol. 3.
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abstract = "Background: Antioxidant supplementation with vitamin E had no effect in the prevention of cardiovascular diseases (CVD) in three recent large, randomized clinical trials. In order to reassess critically the role of vitamin E in CVD prevention, it is important to establish whether these results are related to a lack of antioxidant action. Methods: We examined the in vivo antioxidant effect of vitamin E (300 mg/day for about three years) in 144 participants in the Primary Prevention Project (females and males, aged ≥ 50 y, with at least one major CV risk factor, but no history of CVD). Urinary 8-epi-PGF2α (isoprostane F2α-III or 15-F2t-isoP), a validated biomarker of lipid peroxidation, was measured by mass spectrometry. Results: Urinary excretion of 8-epi-PGF2α [pg/mg creatinine, median (range)] was 141 (67-498) in treated and 148 (76-561) in untreated subjects (p = 0.10). Taking into account possible confounding variables, multiple regression analysis confirmed that vitamin E had no significant effect on this biomarker. Levels of 8-epi-PGF2α were in the normal range for most subjects, except smokers and those with uncontrolled blood pressure or hyperglycemia. Conclusions: Prolonged vitamin E supplementation did not reduce lipid peroxidation in subjects with major cardiovascular risk factors. The observation that the rate of lipid peroxidation was near normal in a large proportion of subjects may help explain why vitamin E was not effective as an antioxidant in the PPP study and was ineffective for CVD prevention in large scale trials.",
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AU - Chiabrando, Chiara

AU - Avanzini, Fausto

AU - Rivalta, Claudia

AU - Colombo, Fabio

AU - Fanelli, Roberto

AU - Palumbo, Gaetana

AU - Roncaglioni, Maria Carla

AU - Pioltelli, Marina Bosisio

AU - Capra, Alberto

AU - Cristofari, Mario

AU - Rossi, Susanna

PY - 2002/3/19

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N2 - Background: Antioxidant supplementation with vitamin E had no effect in the prevention of cardiovascular diseases (CVD) in three recent large, randomized clinical trials. In order to reassess critically the role of vitamin E in CVD prevention, it is important to establish whether these results are related to a lack of antioxidant action. Methods: We examined the in vivo antioxidant effect of vitamin E (300 mg/day for about three years) in 144 participants in the Primary Prevention Project (females and males, aged ≥ 50 y, with at least one major CV risk factor, but no history of CVD). Urinary 8-epi-PGF2α (isoprostane F2α-III or 15-F2t-isoP), a validated biomarker of lipid peroxidation, was measured by mass spectrometry. Results: Urinary excretion of 8-epi-PGF2α [pg/mg creatinine, median (range)] was 141 (67-498) in treated and 148 (76-561) in untreated subjects (p = 0.10). Taking into account possible confounding variables, multiple regression analysis confirmed that vitamin E had no significant effect on this biomarker. Levels of 8-epi-PGF2α were in the normal range for most subjects, except smokers and those with uncontrolled blood pressure or hyperglycemia. Conclusions: Prolonged vitamin E supplementation did not reduce lipid peroxidation in subjects with major cardiovascular risk factors. The observation that the rate of lipid peroxidation was near normal in a large proportion of subjects may help explain why vitamin E was not effective as an antioxidant in the PPP study and was ineffective for CVD prevention in large scale trials.

AB - Background: Antioxidant supplementation with vitamin E had no effect in the prevention of cardiovascular diseases (CVD) in three recent large, randomized clinical trials. In order to reassess critically the role of vitamin E in CVD prevention, it is important to establish whether these results are related to a lack of antioxidant action. Methods: We examined the in vivo antioxidant effect of vitamin E (300 mg/day for about three years) in 144 participants in the Primary Prevention Project (females and males, aged ≥ 50 y, with at least one major CV risk factor, but no history of CVD). Urinary 8-epi-PGF2α (isoprostane F2α-III or 15-F2t-isoP), a validated biomarker of lipid peroxidation, was measured by mass spectrometry. Results: Urinary excretion of 8-epi-PGF2α [pg/mg creatinine, median (range)] was 141 (67-498) in treated and 148 (76-561) in untreated subjects (p = 0.10). Taking into account possible confounding variables, multiple regression analysis confirmed that vitamin E had no significant effect on this biomarker. Levels of 8-epi-PGF2α were in the normal range for most subjects, except smokers and those with uncontrolled blood pressure or hyperglycemia. Conclusions: Prolonged vitamin E supplementation did not reduce lipid peroxidation in subjects with major cardiovascular risk factors. The observation that the rate of lipid peroxidation was near normal in a large proportion of subjects may help explain why vitamin E was not effective as an antioxidant in the PPP study and was ineffective for CVD prevention in large scale trials.

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