TY - JOUR
T1 - Longitudinal tracking of triple labeled umbilical cord derived mesenchymal stromal cells in a mouse model of Amyotrophic Lateral Sclerosis
AU - Violatto, Martina Bruna
AU - Santangelo, Chiara
AU - Capelli, Chiara
AU - Frapolli, Roberta
AU - Ferrari, Raffaele
AU - Sitia, Leopoldo
AU - Tortarolo, Massimo
AU - Talamini, Laura
AU - Previdi, Sara
AU - Moscatelli, Davide
AU - Salmona, Mario
AU - Introna, Martino
AU - Bendotti, Caterina
AU - Bigini, Paolo
PY - 2015/7/1
Y1 - 2015/7/1
N2 - The translational potential of cell therapy to humans requires a deep knowledge of the interaction between transplanted cells and host tissues. In this study, we evaluate the behavior of umbilical cord mesenchymal stromal cells (UC-MSCs), labeled with fluorescent nanoparticles, transplanted in healthy or early symptomatic transgenic SOD1G93A mice (a murine model of Amyotrophic Lateral Sclerosis). The double labeling of cells with nanoparticles and Hoechst-33258 enabled their tracking for a long time in both cells and tissues. Whole-body distribution of UC-MSCs was performed by in-vivo and ex-vivo analyses 1, 7, 21. days after single intravenous or intracerebroventricular administration. By intravenous administration cells were sequestered by the lungs and rapidly cleared by the liver. No difference in biodistribution was found among the two groups. On the other hand, UC-MSCs transplanted in lateral ventricles remained on the choroid plexus for the whole duration of the study even if decreasing in number. Few cells were found in the spinal cord of SOD1G93A mice exclusively. No migration in brain parenchyma was observed. These results suggest that the direct implantation in brain ventricles allows a prolonged permanence of cells close to the damaged areas and makes this method of tracking reliable for future studies of efficacy.
AB - The translational potential of cell therapy to humans requires a deep knowledge of the interaction between transplanted cells and host tissues. In this study, we evaluate the behavior of umbilical cord mesenchymal stromal cells (UC-MSCs), labeled with fluorescent nanoparticles, transplanted in healthy or early symptomatic transgenic SOD1G93A mice (a murine model of Amyotrophic Lateral Sclerosis). The double labeling of cells with nanoparticles and Hoechst-33258 enabled their tracking for a long time in both cells and tissues. Whole-body distribution of UC-MSCs was performed by in-vivo and ex-vivo analyses 1, 7, 21. days after single intravenous or intracerebroventricular administration. By intravenous administration cells were sequestered by the lungs and rapidly cleared by the liver. No difference in biodistribution was found among the two groups. On the other hand, UC-MSCs transplanted in lateral ventricles remained on the choroid plexus for the whole duration of the study even if decreasing in number. Few cells were found in the spinal cord of SOD1G93A mice exclusively. No migration in brain parenchyma was observed. These results suggest that the direct implantation in brain ventricles allows a prolonged permanence of cells close to the damaged areas and makes this method of tracking reliable for future studies of efficacy.
KW - Amyotrophic Lateral Sclerosis
KW - Cell tracking
KW - In vivo imaging
KW - Mesenchymal stromal cells
KW - Nanotechnology
KW - Umbilical cord
UR - http://www.scopus.com/inward/record.url?scp=84938584853&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84938584853&partnerID=8YFLogxK
U2 - 10.1016/j.scr.2015.06.010
DO - 10.1016/j.scr.2015.06.010
M3 - Article
C2 - 26177481
AN - SCOPUS:84938584853
VL - 15
SP - 243
EP - 253
JO - Stem Cell Research
JF - Stem Cell Research
SN - 1873-5061
IS - 1
ER -