Loss of function of miR-342-3p results in MCT1 over-expression and contributes to oncogenic metabolic reprogramming in triple negative breast cancer

Sandra L Romero-Cordoba, Sergio Rodriguez-Cuevas, Veronica Bautista-Pina, Antonio Maffuz-Aziz, Elvira D'Ippolito, Giulia Cosentino, Sara Baroni, Marilena V Iorio, Alfredo Hidalgo-Miranda

Research output: Contribution to journalArticle

Abstract

Triple-negative breast cancer (TNBC) is a heterogeneous and aggressive neoplasia lacking the expression of hormonal receptors and human epidermal growth factor receptor-2. Accumulating evidence has highlighted the importance of miRNAs dysregulation in the establishment of cancer programs, but the functional role of many miRNAs remains unclear. The description of miRNAs roles might provide novel strategies for treatment. In the present work, an integrated analysis of miRNA transcriptional landscape was performed (N = 132), identifying the significant down-modulation of miR-342-3p in TNBC, probably because of the aberrant activity of estrogen receptor, which serves as a transcription factor of the miRNA, as demonstrated by a siRNA-knockdown approach. The enhanced expression of miR-342-3p significantly decreased cell proliferation, viability and migration rates of diverse TN cells in vitro. Bioinformatic and functional analyses revealed that miR-342-3p directly targets the monocarboxylate transporter 1 (MCT1), which promotes lactate and glucose fluxes alteration, thus disrupting the metabolic homeostasis of tumor cells. Optical metabolic imaging assay defined a higher optical redox ratio in glycolytic cells overexpressing miR-342-3p. Furthermore, we found that hypoxic conditions and glucose starvation attenuate miR-342-3p expression, suggesting a crosstalk program between these metabolic factors. Consistently, miR-342-3p down-modulation is associated with an increased MCT1 expression level and glycolytic score in human triple negative tumors. Overall, we described for the first time the regulatory activity of miR-342-3p on relevant metabolic carcinogenic pathways in TN breast cancers.

Original languageEnglish
Pages (from-to)12252
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - Aug 16 2018

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Triple Negative Breast Neoplasms
MicroRNAs
Neoplasms
Glucose
Optical Imaging
Starvation
Metabolic Networks and Pathways
Computational Biology
Estrogen Receptors
Small Interfering RNA
Oxidation-Reduction
Cell Movement
Lactic Acid
Cell Survival
Homeostasis
Transcription Factors
Cell Proliferation
Breast Neoplasms

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Loss of function of miR-342-3p results in MCT1 over-expression and contributes to oncogenic metabolic reprogramming in triple negative breast cancer. / Romero-Cordoba, Sandra L; Rodriguez-Cuevas, Sergio; Bautista-Pina, Veronica; Maffuz-Aziz, Antonio; D'Ippolito, Elvira; Cosentino, Giulia; Baroni, Sara; Iorio, Marilena V; Hidalgo-Miranda, Alfredo.

In: Scientific Reports, Vol. 8, No. 1, 16.08.2018, p. 12252.

Research output: Contribution to journalArticle

Romero-Cordoba, SL, Rodriguez-Cuevas, S, Bautista-Pina, V, Maffuz-Aziz, A, D'Ippolito, E, Cosentino, G, Baroni, S, Iorio, MV & Hidalgo-Miranda, A 2018, 'Loss of function of miR-342-3p results in MCT1 over-expression and contributes to oncogenic metabolic reprogramming in triple negative breast cancer', Scientific Reports, vol. 8, no. 1, pp. 12252. https://doi.org/10.1038/s41598-018-29708-9
Romero-Cordoba, Sandra L ; Rodriguez-Cuevas, Sergio ; Bautista-Pina, Veronica ; Maffuz-Aziz, Antonio ; D'Ippolito, Elvira ; Cosentino, Giulia ; Baroni, Sara ; Iorio, Marilena V ; Hidalgo-Miranda, Alfredo. / Loss of function of miR-342-3p results in MCT1 over-expression and contributes to oncogenic metabolic reprogramming in triple negative breast cancer. In: Scientific Reports. 2018 ; Vol. 8, No. 1. pp. 12252.
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AU - D'Ippolito, Elvira

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AB - Triple-negative breast cancer (TNBC) is a heterogeneous and aggressive neoplasia lacking the expression of hormonal receptors and human epidermal growth factor receptor-2. Accumulating evidence has highlighted the importance of miRNAs dysregulation in the establishment of cancer programs, but the functional role of many miRNAs remains unclear. The description of miRNAs roles might provide novel strategies for treatment. In the present work, an integrated analysis of miRNA transcriptional landscape was performed (N = 132), identifying the significant down-modulation of miR-342-3p in TNBC, probably because of the aberrant activity of estrogen receptor, which serves as a transcription factor of the miRNA, as demonstrated by a siRNA-knockdown approach. The enhanced expression of miR-342-3p significantly decreased cell proliferation, viability and migration rates of diverse TN cells in vitro. Bioinformatic and functional analyses revealed that miR-342-3p directly targets the monocarboxylate transporter 1 (MCT1), which promotes lactate and glucose fluxes alteration, thus disrupting the metabolic homeostasis of tumor cells. Optical metabolic imaging assay defined a higher optical redox ratio in glycolytic cells overexpressing miR-342-3p. Furthermore, we found that hypoxic conditions and glucose starvation attenuate miR-342-3p expression, suggesting a crosstalk program between these metabolic factors. Consistently, miR-342-3p down-modulation is associated with an increased MCT1 expression level and glycolytic score in human triple negative tumors. Overall, we described for the first time the regulatory activity of miR-342-3p on relevant metabolic carcinogenic pathways in TN breast cancers.

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