Abstract
Neuroblastoma is characterized by non-random chromosome 1p deletion. About 70% of human neuroblastomas in advanced stages show loss of the short arm of chromosome 1. Moreover, the remaining neuroblastomas can have molecular deletion that are not detected by cytogenetic analysis. We studied loss of heterozigosity (LOH) on chromosome 1 in 26 neuroblastoma tissues belonging to patients in different stages of disease employing probes derived from microdissection and microcloning of the short arm of chromosome 1. LOH was found both in patients in stage 1 and in stage 4. Patients in stage 4 show consistent deletion at locus D1S94 corresponding to the chromosomal region 1p36.11-1p36.12. In this region could be located the putative neuroblastoma supressor gene. Combined analysis of LOH and MYCN gene amplification does not show relation between these two molecular abnormalities.
| Original language | English |
|---|---|
| Pages (from-to) | 227-231 |
| Number of pages | 5 |
| Journal | Clinical Chemistry and Enzymology Communications |
| Volume | 5 |
| Issue number | 4-6 |
| Publication status | Published - 1993 |
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Keywords
- Chromosome 1
- Loss-of-heterozigosity
- MYCN oncogene
- Neuroblastoma
ASJC Scopus subject areas
- Clinical Biochemistry
Cite this
Loss of heterozigosity for 1p chromosome in neuroblastoma. / Valenti, C.; Di Martino, D.; De Bernardi, B.; Garaventa, A.; Bottini, F.; Tonini, G. P.
In: Clinical Chemistry and Enzymology Communications, Vol. 5, No. 4-6, 1993, p. 227-231.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Loss of heterozigosity for 1p chromosome in neuroblastoma
AU - Valenti, C.
AU - Di Martino, D.
AU - De Bernardi, B.
AU - Garaventa, A.
AU - Bottini, F.
AU - Tonini, G. P.
PY - 1993
Y1 - 1993
N2 - Neuroblastoma is characterized by non-random chromosome 1p deletion. About 70% of human neuroblastomas in advanced stages show loss of the short arm of chromosome 1. Moreover, the remaining neuroblastomas can have molecular deletion that are not detected by cytogenetic analysis. We studied loss of heterozigosity (LOH) on chromosome 1 in 26 neuroblastoma tissues belonging to patients in different stages of disease employing probes derived from microdissection and microcloning of the short arm of chromosome 1. LOH was found both in patients in stage 1 and in stage 4. Patients in stage 4 show consistent deletion at locus D1S94 corresponding to the chromosomal region 1p36.11-1p36.12. In this region could be located the putative neuroblastoma supressor gene. Combined analysis of LOH and MYCN gene amplification does not show relation between these two molecular abnormalities.
AB - Neuroblastoma is characterized by non-random chromosome 1p deletion. About 70% of human neuroblastomas in advanced stages show loss of the short arm of chromosome 1. Moreover, the remaining neuroblastomas can have molecular deletion that are not detected by cytogenetic analysis. We studied loss of heterozigosity (LOH) on chromosome 1 in 26 neuroblastoma tissues belonging to patients in different stages of disease employing probes derived from microdissection and microcloning of the short arm of chromosome 1. LOH was found both in patients in stage 1 and in stage 4. Patients in stage 4 show consistent deletion at locus D1S94 corresponding to the chromosomal region 1p36.11-1p36.12. In this region could be located the putative neuroblastoma supressor gene. Combined analysis of LOH and MYCN gene amplification does not show relation between these two molecular abnormalities.
KW - Chromosome 1
KW - Loss-of-heterozigosity
KW - MYCN oncogene
KW - Neuroblastoma
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UR - http://www.scopus.com/inward/citedby.url?scp=0027745615&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0027745615
VL - 5
SP - 227
EP - 231
JO - Clinical Chemistry and Enzymology Communications
JF - Clinical Chemistry and Enzymology Communications
SN - 0892-2187
IS - 4-6
ER -