Loss of heterozigosity for 1p chromosome in neuroblastoma

C. Valenti; D. Di Martino; B. De Bernardi; A. Garaventa; F. Bottini; G. P. Tonini

Loss of heterozigosity for 1p chromosome in neuroblastoma

C. Valenti, D. Di Martino, B. De Bernardi, A. Garaventa, F. Bottini, G. P. Tonini

Istituto "Giannina Gaslini"

Research output: Contribution to journal › Article › peer-review

Abstract

Neuroblastoma is characterized by non-random chromosome 1p deletion. About 70% of human neuroblastomas in advanced stages show loss of the short arm of chromosome 1. Moreover, the remaining neuroblastomas can have molecular deletion that are not detected by cytogenetic analysis. We studied loss of heterozigosity (LOH) on chromosome 1 in 26 neuroblastoma tissues belonging to patients in different stages of disease employing probes derived from microdissection and microcloning of the short arm of chromosome 1. LOH was found both in patients in stage 1 and in stage 4. Patients in stage 4 show consistent deletion at locus D1S94 corresponding to the chromosomal region 1p36.11-1p36.12. In this region could be located the putative neuroblastoma supressor gene. Combined analysis of LOH and MYCN gene amplification does not show relation between these two molecular abnormalities.

Original language	English
Pages (from-to)	227-231
Number of pages	5
Journal	Clinical Chemistry and Enzymology Communications
Volume	5
Issue number	4-6
Publication status	Published - 1993

Keywords

Chromosome 1
Loss-of-heterozigosity
MYCN oncogene
Neuroblastoma

ASJC Scopus subject areas

Clinical Biochemistry

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abstract = "Neuroblastoma is characterized by non-random chromosome 1p deletion. About 70% of human neuroblastomas in advanced stages show loss of the short arm of chromosome 1. Moreover, the remaining neuroblastomas can have molecular deletion that are not detected by cytogenetic analysis. We studied loss of heterozigosity (LOH) on chromosome 1 in 26 neuroblastoma tissues belonging to patients in different stages of disease employing probes derived from microdissection and microcloning of the short arm of chromosome 1. LOH was found both in patients in stage 1 and in stage 4. Patients in stage 4 show consistent deletion at locus D1S94 corresponding to the chromosomal region 1p36.11-1p36.12. In this region could be located the putative neuroblastoma supressor gene. Combined analysis of LOH and MYCN gene amplification does not show relation between these two molecular abnormalities.",

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T1 - Loss of heterozigosity for 1p chromosome in neuroblastoma

AU - Valenti, C.

AU - Di Martino, D.

AU - De Bernardi, B.

AU - Garaventa, A.

AU - Bottini, F.

AU - Tonini, G. P.

PY - 1993

Y1 - 1993

N2 - Neuroblastoma is characterized by non-random chromosome 1p deletion. About 70% of human neuroblastomas in advanced stages show loss of the short arm of chromosome 1. Moreover, the remaining neuroblastomas can have molecular deletion that are not detected by cytogenetic analysis. We studied loss of heterozigosity (LOH) on chromosome 1 in 26 neuroblastoma tissues belonging to patients in different stages of disease employing probes derived from microdissection and microcloning of the short arm of chromosome 1. LOH was found both in patients in stage 1 and in stage 4. Patients in stage 4 show consistent deletion at locus D1S94 corresponding to the chromosomal region 1p36.11-1p36.12. In this region could be located the putative neuroblastoma supressor gene. Combined analysis of LOH and MYCN gene amplification does not show relation between these two molecular abnormalities.

AB - Neuroblastoma is characterized by non-random chromosome 1p deletion. About 70% of human neuroblastomas in advanced stages show loss of the short arm of chromosome 1. Moreover, the remaining neuroblastomas can have molecular deletion that are not detected by cytogenetic analysis. We studied loss of heterozigosity (LOH) on chromosome 1 in 26 neuroblastoma tissues belonging to patients in different stages of disease employing probes derived from microdissection and microcloning of the short arm of chromosome 1. LOH was found both in patients in stage 1 and in stage 4. Patients in stage 4 show consistent deletion at locus D1S94 corresponding to the chromosomal region 1p36.11-1p36.12. In this region could be located the putative neuroblastoma supressor gene. Combined analysis of LOH and MYCN gene amplification does not show relation between these two molecular abnormalities.

KW - Chromosome 1

KW - Loss-of-heterozigosity

KW - MYCN oncogene

KW - Neuroblastoma

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