Neuroblastoma is characterized by non-random chromosome 1p deletion. About 70% of human neuroblastomas in advanced stages show loss of the short arm of chromosome 1. Moreover, the remaining neuroblastomas can have molecular deletion that are not detected by cytogenetic analysis. We studied loss of heterozigosity (LOH) on chromosome 1 in 26 neuroblastoma tissues belonging to patients in different stages of disease employing probes derived from microdissection and microcloning of the short arm of chromosome 1. LOH was found both in patients in stage 1 and in stage 4. Patients in stage 4 show consistent deletion at locus D1S94 corresponding to the chromosomal region 1p36.11-1p36.12. In this region could be located the putative neuroblastoma supressor gene. Combined analysis of LOH and MYCN gene amplification does not show relation between these two molecular abnormalities.
|Number of pages||5|
|Journal||Clinical Chemistry and Enzymology Communications|
|Publication status||Published - 1993|
- Chromosome 1
- MYCN oncogene
ASJC Scopus subject areas
- Clinical Biochemistry