Loss of polymorphic restriction fragments of class I and class II MHC genes in a malignant melanoma

G. Angelini, G. Fossati, P. Radice, A. Longo, P. Piccioli, M. A. Pierotti, G. B. Ferrara, G. Parmiani

Research output: Contribution to journalArticle

Abstract

DNAs from human malignant melanoma cells and autologous peripheral blood lymphocytes were evaluated by Southern blot analysis with probes for class I and II HLA genes. DNA of melanoma cells digested with PvuII, EcoRI and Bg1I and hybridized with a DRβ probe showed a loss of several fragments when compared with DNA from lymphocytes. The same DNAs were not distinguishable when hybridized with a DQβ probe. Analysis of melanoma and autologous lymphocyte DNAs from the same patient with a class I cDNA, after digestion with several endonucleases, revealed a further loss of fragments in melanoma cells. Comparison of restriction fragment patterns of melanoma and lymphocytes with those of homozygous, serologically-typed cell lines indicated that melanoma cells have lost fragments diagnostic of DR2 and A1 antigens. A densitometric analysis of signals of several oncogenes in comparison with that of DR indicated that a duplication of the remaining DR allele had occurred in melanoma cells.

Original languageEnglish
Pages (from-to)241-246
Number of pages6
JournalJournal of Immunogenetics
Volume13
Issue number2-3
Publication statusPublished - 1986

Fingerprint

MHC Class II Genes
Melanoma
Lymphocytes
DNA
MHC Class I Genes
Endonucleases
Southern Blotting
Oncogenes
Digestion
Complementary DNA
Alleles
Antigens
Cell Line

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Angelini, G., Fossati, G., Radice, P., Longo, A., Piccioli, P., Pierotti, M. A., ... Parmiani, G. (1986). Loss of polymorphic restriction fragments of class I and class II MHC genes in a malignant melanoma. Journal of Immunogenetics, 13(2-3), 241-246.

Loss of polymorphic restriction fragments of class I and class II MHC genes in a malignant melanoma. / Angelini, G.; Fossati, G.; Radice, P.; Longo, A.; Piccioli, P.; Pierotti, M. A.; Ferrara, G. B.; Parmiani, G.

In: Journal of Immunogenetics, Vol. 13, No. 2-3, 1986, p. 241-246.

Research output: Contribution to journalArticle

Angelini, G, Fossati, G, Radice, P, Longo, A, Piccioli, P, Pierotti, MA, Ferrara, GB & Parmiani, G 1986, 'Loss of polymorphic restriction fragments of class I and class II MHC genes in a malignant melanoma', Journal of Immunogenetics, vol. 13, no. 2-3, pp. 241-246.
Angelini, G. ; Fossati, G. ; Radice, P. ; Longo, A. ; Piccioli, P. ; Pierotti, M. A. ; Ferrara, G. B. ; Parmiani, G. / Loss of polymorphic restriction fragments of class I and class II MHC genes in a malignant melanoma. In: Journal of Immunogenetics. 1986 ; Vol. 13, No. 2-3. pp. 241-246.
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AU - Angelini, G.

AU - Fossati, G.

AU - Radice, P.

AU - Longo, A.

AU - Piccioli, P.

AU - Pierotti, M. A.

AU - Ferrara, G. B.

AU - Parmiani, G.

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AB - DNAs from human malignant melanoma cells and autologous peripheral blood lymphocytes were evaluated by Southern blot analysis with probes for class I and II HLA genes. DNA of melanoma cells digested with PvuII, EcoRI and Bg1I and hybridized with a DRβ probe showed a loss of several fragments when compared with DNA from lymphocytes. The same DNAs were not distinguishable when hybridized with a DQβ probe. Analysis of melanoma and autologous lymphocyte DNAs from the same patient with a class I cDNA, after digestion with several endonucleases, revealed a further loss of fragments in melanoma cells. Comparison of restriction fragment patterns of melanoma and lymphocytes with those of homozygous, serologically-typed cell lines indicated that melanoma cells have lost fragments diagnostic of DR2 and A1 antigens. A densitometric analysis of signals of several oncogenes in comparison with that of DR indicated that a duplication of the remaining DR allele had occurred in melanoma cells.

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