Loss of polymorphic restriction fragments of class I and class II MHC genes in a malignant melanoma

G. Angelini, G. Fossati, P. Radice, A. Longo, P. Piccioli, M. A. Pierotti, G. B. Ferrara, G. Parmiani

Research output: Contribution to journalArticlepeer-review

Abstract

DNAs from human malignant melanoma cells and autologous peripheral blood lymphocytes were evaluated by Southern blot analysis with probes for class I and II HLA genes. DNA of melanoma cells digested with PvuII, EcoRI and Bg1I and hybridized with a DRβ probe showed a loss of several fragments when compared with DNA from lymphocytes. The same DNAs were not distinguishable when hybridized with a DQβ probe. Analysis of melanoma and autologous lymphocyte DNAs from the same patient with a class I cDNA, after digestion with several endonucleases, revealed a further loss of fragments in melanoma cells. Comparison of restriction fragment patterns of melanoma and lymphocytes with those of homozygous, serologically-typed cell lines indicated that melanoma cells have lost fragments diagnostic of DR2 and A1 antigens. A densitometric analysis of signals of several oncogenes in comparison with that of DR indicated that a duplication of the remaining DR allele had occurred in melanoma cells.

Original languageEnglish
Pages (from-to)241-246
Number of pages6
JournalJournal of Immunogenetics
Volume13
Issue number2-3
Publication statusPublished - 1986

ASJC Scopus subject areas

  • Immunology
  • Genetics

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