Loss of the canonical spindle orientation function in the Pins/LGN homolog AGS3

Mehdi Saadaoui, Daijiro Konno, Karine Loulier, Rosette Goiame, Vaibhav Jadhav, Marina Mapelli, Fumio Matsuzaki, Xavier Morin

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In many cell types, mitotic spindle orientation relies on the canonical “LGN complex” composed of Pins/LGN, Mud/NuMA, and Gαi subunits. Membrane localization of this complex recruits motor force generators that pull on astral microtubules to orient the spindle. Drosophila Pins shares highly conserved functional domains with its two vertebrate homologs LGN and AGS3. Whereas the role of Pins and LGN in oriented divisions is extensively documented, involvement of AGS3 remains controversial. Here, we show that AGS3 is not required for planar divisions of neural progenitors in the mouse neocortex. AGS3 is not recruited to the cell cortex and does not rescue LGN loss of function. Despite conserved interactions with NuMA and Gαi in vitro, comparison of LGN and AGS3 functional domains in vivo reveals unexpected differences in the ability of these interactions to mediate spindle orientation functions. Finally, we find that Drosophila Pins is unable to substitute for LGN loss of function in vertebrates, highlighting that species-specific modulations of the interactions between components of the Pins/LGN complex are crucial in vivo for spindle orientation.

Original languageEnglish
Pages (from-to)1509-1520
Number of pages12
JournalEMBO Reports
Volume18
Issue number9
DOIs
Publication statusPublished - Sep 1 2017

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Drosophila
Vertebrates
Spindle Apparatus
Neocortex
Microtubules
Modulation
Membranes
In Vitro Techniques

Keywords

  • AGS3
  • LGN
  • Pins
  • spindle orientation
  • vertebrate neuroepithelium

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Saadaoui, M., Konno, D., Loulier, K., Goiame, R., Jadhav, V., Mapelli, M., ... Morin, X. (2017). Loss of the canonical spindle orientation function in the Pins/LGN homolog AGS3. EMBO Reports, 18(9), 1509-1520. https://doi.org/10.15252/embr.201643048

Loss of the canonical spindle orientation function in the Pins/LGN homolog AGS3. / Saadaoui, Mehdi; Konno, Daijiro; Loulier, Karine; Goiame, Rosette; Jadhav, Vaibhav; Mapelli, Marina; Matsuzaki, Fumio; Morin, Xavier.

In: EMBO Reports, Vol. 18, No. 9, 01.09.2017, p. 1509-1520.

Research output: Contribution to journalArticle

Saadaoui, M, Konno, D, Loulier, K, Goiame, R, Jadhav, V, Mapelli, M, Matsuzaki, F & Morin, X 2017, 'Loss of the canonical spindle orientation function in the Pins/LGN homolog AGS3', EMBO Reports, vol. 18, no. 9, pp. 1509-1520. https://doi.org/10.15252/embr.201643048
Saadaoui, Mehdi ; Konno, Daijiro ; Loulier, Karine ; Goiame, Rosette ; Jadhav, Vaibhav ; Mapelli, Marina ; Matsuzaki, Fumio ; Morin, Xavier. / Loss of the canonical spindle orientation function in the Pins/LGN homolog AGS3. In: EMBO Reports. 2017 ; Vol. 18, No. 9. pp. 1509-1520.
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