Loss of transcriptional control over endogenous retroelements during reprogramming to pluripotency

Marc Friedli, Priscilla Turelli, Adamandia Kapopoulou, Benjamin Rauwel, Nathaly Castro-Diáz, Helen M. Rowe, Gabriela Ecco, Carmen Unzu, Evarist Planet, Angelo Lombardo, Bastien Mangeat, Barbara E. Wildhaber, Luigi Naldini, Didier Trono

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Endogenous retroelements (EREs) account for about half of the mouse or human genome, and their potential as insertional mutagens and transcriptional perturbators is suppressed by early embryonic epigenetic silencing. Here, we asked how ERE control is maintained during the generation of induced pluripotent stem cells (iPSCs), as this procedure involves profound epigenetic remodeling. We found that all EREs tested were markedly up-regulated during the reprogramming of either mouse embryonic fibroblasts, human CD34+ cells, or human primary hepatocytes. At the iPSC stage, EREs of some classes were repressed, whereas others remained highly expressed, yielding a pattern somewhat reminiscent of that recorded in embryonic stem cells. However, variability persisted between individual iPSC clones in the control of specific ERE integrants. Both during reprogramming and in iPS cells, the up-regulation of specific EREs significantly impacted on the transcription of nearby cellular genes. While transcription triggered by specific ERE integrants at highly precise developmental stages may be an essential step toward obtaining pluripotent cells, the broad and unspecific unleashing of the repetitive genome observed here may contribute to the inefficiency of the reprogramming process and to the phenotypic heterogeneity of iPSCs.

Original languageEnglish
Pages (from-to)1251-1259
Number of pages9
JournalGenome Research
Volume24
Issue number8
DOIs
Publication statusPublished - 2014

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Retroelements
Induced Pluripotent Stem Cells
Epigenomics
Mutagens
Human Genome
Embryonic Stem Cells
Hepatocytes
Up-Regulation
Clone Cells
Fibroblasts
Genome
Genes

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Friedli, M., Turelli, P., Kapopoulou, A., Rauwel, B., Castro-Diáz, N., Rowe, H. M., ... Trono, D. (2014). Loss of transcriptional control over endogenous retroelements during reprogramming to pluripotency. Genome Research, 24(8), 1251-1259. https://doi.org/10.1101/gr.172809.114

Loss of transcriptional control over endogenous retroelements during reprogramming to pluripotency. / Friedli, Marc; Turelli, Priscilla; Kapopoulou, Adamandia; Rauwel, Benjamin; Castro-Diáz, Nathaly; Rowe, Helen M.; Ecco, Gabriela; Unzu, Carmen; Planet, Evarist; Lombardo, Angelo; Mangeat, Bastien; Wildhaber, Barbara E.; Naldini, Luigi; Trono, Didier.

In: Genome Research, Vol. 24, No. 8, 2014, p. 1251-1259.

Research output: Contribution to journalArticle

Friedli, M, Turelli, P, Kapopoulou, A, Rauwel, B, Castro-Diáz, N, Rowe, HM, Ecco, G, Unzu, C, Planet, E, Lombardo, A, Mangeat, B, Wildhaber, BE, Naldini, L & Trono, D 2014, 'Loss of transcriptional control over endogenous retroelements during reprogramming to pluripotency', Genome Research, vol. 24, no. 8, pp. 1251-1259. https://doi.org/10.1101/gr.172809.114
Friedli M, Turelli P, Kapopoulou A, Rauwel B, Castro-Diáz N, Rowe HM et al. Loss of transcriptional control over endogenous retroelements during reprogramming to pluripotency. Genome Research. 2014;24(8):1251-1259. https://doi.org/10.1101/gr.172809.114
Friedli, Marc ; Turelli, Priscilla ; Kapopoulou, Adamandia ; Rauwel, Benjamin ; Castro-Diáz, Nathaly ; Rowe, Helen M. ; Ecco, Gabriela ; Unzu, Carmen ; Planet, Evarist ; Lombardo, Angelo ; Mangeat, Bastien ; Wildhaber, Barbara E. ; Naldini, Luigi ; Trono, Didier. / Loss of transcriptional control over endogenous retroelements during reprogramming to pluripotency. In: Genome Research. 2014 ; Vol. 24, No. 8. pp. 1251-1259.
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