Loss of Y chromosome with retention of Y heterochromatin in a marker chromosome from a human melanoma

L. Doneda, L. Larizza

Research output: Contribution to journalArticlepeer-review

Abstract

A single copy of a der15 chromosome (m3) characterized by a C- and distamycin A-Dapi-positive region was observed in the -Y hyperploid karyotype of a primary human melanoma (Me 1402). The heterochromatic region was located pericentromerically, adjacent at one end to the NOR region of chromosome 15, and at the other to an unclassifiable chromosomal piece. We established that the C-positive block in the marker chromosome originated from Y heterochromatin by high-stringency in situ hybridization with a DNA probe for the 2.1 Hae III Y-specific repeat. Loss of the Y chromosome in tumors has been considered to be a secondary event associated with malignant evolution. It is significant that Me 1402 cells, which are highly malignant, lack the Y chromosome, but retain its heterochromatic portion in the rearranged m3 chromosome.

Original languageEnglish
Pages (from-to)154-157
Number of pages4
JournalInternational Journal of Cancer
Volume47
Issue number1
DOIs
Publication statusPublished - 1991

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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