Loss or altered subcellular localization of p27 in Barrett's associated adenocarcinoma

Surendra P. Singh, Jennifer Lipman, Harvey Goldman, F. Henry Ellis, Laura Aizenman, M. Giulia Cangi, Sabina Signoretti, Dah S. Chiaur, Michele Pagano, Massimo Loda

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Abstract

The cyclin-dependent kinase inhibitor p27 is a negative regulator of the cell division cycle. It is expressed at the highest levels during the quiescent (G0) and prereplicative (G1) phases, and its degradation is required for entry into the S phase. Because lack of p27 is associated with aggressive behavior in a variety of tumors of epithelial and lymphoid origin, we used immunohistochemistry and in situ hybridization to evaluate the expression of p27 in metaplastic and dysplastic Barrett's epithelium and to assess its prognostic significance in Barrett's associated adenocarcinoma (BAA) of the esophagus. In metaplastic Barrett's epithelium, p27 protein and mRNA were restricted to the superficial third of glands in all cases and extended to the lower third in 4 cases. In contrast, expression of p27 message and protein was both increased and full-thickness, in the 23 cases with high-grade dysplasia adjacent to BAA and in carcinoma in situ. Although all invasive carcinomas had elevated levels of p27 mRNA, 45 (83%) of 54 invasive carcinomas had low p27 protein levels (

Original languageEnglish
Pages (from-to)1730-1735
Number of pages6
JournalCancer Research
Volume58
Issue number8
Publication statusPublished - Apr 15 1998

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ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Singh, S. P., Lipman, J., Goldman, H., Ellis, F. H., Aizenman, L., Cangi, M. G., Signoretti, S., Chiaur, D. S., Pagano, M., & Loda, M. (1998). Loss or altered subcellular localization of p27 in Barrett's associated adenocarcinoma. Cancer Research, 58(8), 1730-1735.