Losses of heterozygosity in oral and oropharyngeal epithelial carcinomas

Francesca R. Grati, Silvia M. Sirchia, Isabella Garagiola, Elena Sironi, Silvestre Galioto, Franca Rossella, Paola Serafini, Francesca Dulcetti, Alberto Bozzetti, Roberto Brusati, Giuseppe Simoni

Research output: Contribution to journalArticle

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Abstract

We analyzed 25 oral and oropharyngeal epithelial carcinomas for loss of heterozygosity (LOH) and microsatellite instability by using 55 oligonucleotide repeat markers located in 45 chromosomal regions. The aim was to identify which chromosomal regions and tumor-suppressor genes (TSGs) are preferentially lost in these tumors and to relate LOH at specific loci to clinicopathologic data. The analysis was performed on tumor tissue and on a corresponding normal tissue (blood lymphocytes) with the use of the polymerase chain reaction technique followed by microsatellite allele separation with denaturing gel electrophoresis. Thirty-two of 45 chromosomal regions demonstrated a significant (≥20%) incidence of LOH. An allelic loss of ≥50% was found in 9p21 (77.8%), 8p22-23 (70%), 3p12 (61.5%), 1p36.1 and 12q22 (60%), 3q28 (57.1%), 5q23.3 (54.5%), 3p25-26, 3p24, and 7q35 (50%). We did not find any microsatellite instability. Our results suggest that in addition to a group of TSGs, pleiotropic for several tumor types, other suppressor genes are specifically involved in oral and oropharyngeal carcinogenesis. Copyright (C) 2000 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)57-61
Number of pages5
JournalCancer Genetics and Cytogenetics
Volume118
Issue number1
DOIs
Publication statusPublished - Apr 1 2000

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Loss of Heterozygosity
Tumor Suppressor Genes
Carcinoma
Microsatellite Instability
Oligonucleotides
Microsatellite Repeats
Electrophoresis
Neoplasms
Carcinogenesis
Gels
Alleles
Lymphocytes
Polymerase Chain Reaction
Incidence

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Losses of heterozygosity in oral and oropharyngeal epithelial carcinomas. / Grati, Francesca R.; Sirchia, Silvia M.; Garagiola, Isabella; Sironi, Elena; Galioto, Silvestre; Rossella, Franca; Serafini, Paola; Dulcetti, Francesca; Bozzetti, Alberto; Brusati, Roberto; Simoni, Giuseppe.

In: Cancer Genetics and Cytogenetics, Vol. 118, No. 1, 01.04.2000, p. 57-61.

Research output: Contribution to journalArticle

Grati, FR, Sirchia, SM, Garagiola, I, Sironi, E, Galioto, S, Rossella, F, Serafini, P, Dulcetti, F, Bozzetti, A, Brusati, R & Simoni, G 2000, 'Losses of heterozygosity in oral and oropharyngeal epithelial carcinomas', Cancer Genetics and Cytogenetics, vol. 118, no. 1, pp. 57-61. https://doi.org/10.1016/S0165-4608(99)00199-5
Grati, Francesca R. ; Sirchia, Silvia M. ; Garagiola, Isabella ; Sironi, Elena ; Galioto, Silvestre ; Rossella, Franca ; Serafini, Paola ; Dulcetti, Francesca ; Bozzetti, Alberto ; Brusati, Roberto ; Simoni, Giuseppe. / Losses of heterozygosity in oral and oropharyngeal epithelial carcinomas. In: Cancer Genetics and Cytogenetics. 2000 ; Vol. 118, No. 1. pp. 57-61.
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