Low Ca2+-sensitive maxi-K+ channels in human cultured fibroblasts

Luis J V Galietta, Zygmunt Galdzicki, Mario Nobile

Research output: Contribution to journalArticle

Abstract

The patch clamp technique was used to reveal single channel activity in the membrane of human cultured fibroblasts. The most frequently detected ion channel type was a Ca2+-dependent K+ channel with a conductance of 287±38 pS in symmetrical 130 mM KCl. The channel showed a peculiar low Ca2+-sensitivity compared to that of similar channels in other preparations. In fact micromolar values of internal Ca2+ were not effective in the channel activation, except at high depolarizing membrane potentials. The activity was highly increased only when the channel was exposed to relatively high internal Ca2+ concentrations (0.2-2.0 mM).

Original languageEnglish
Pages (from-to)99-101
Number of pages3
JournalPflugers Archiv European Journal of Physiology
Volume413
Issue number1
DOIs
Publication statusPublished - Nov 1988

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Large-Conductance Calcium-Activated Potassium Channels
Patch-Clamp Techniques
Fibroblasts
Ion Channels
Membrane Potentials
Membranes
Clamping devices
Chemical activation

Keywords

  • Ca-sensitivity
  • human fibroblasts
  • maxi-K channels
  • patch-clamp technique

ASJC Scopus subject areas

  • Physiology

Cite this

Low Ca2+-sensitive maxi-K+ channels in human cultured fibroblasts. / Galietta, Luis J V; Galdzicki, Zygmunt; Nobile, Mario.

In: Pflugers Archiv European Journal of Physiology, Vol. 413, No. 1, 11.1988, p. 99-101.

Research output: Contribution to journalArticle

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