Low CD4 counts rather than superantigenic-like effects account for differences in expressed T-cell receptor (TCR) repertoires between HIV-1 seropositive long-term non-progressors and individuals with progressive disease

Michael Westby, Andrew N. Vaughan, Claudia Balotta, Massimo Galli, Mario Clerici, Angus G. Dalgleish

Research output: Contribution to journalArticle

Abstract

Analysis of HIV-infected individuals who have stable CD4 counts many years after seroconversion may provide an insight as to how the host's immune system can successfully control HIV infection. In this study we analysed the T-cell receptor (TCR) Vβ repertoire in 13 HIV+ individuals, seven of whom were classed as long-term non-progressors (LTNP), using a technique which couples anchor PCR (AnPCR) amplification of β-chain cDNA to differential probe hybridization with non-radioactively labelled Vβ family specific oligonucleotide probes. There were no significant differences in the expressed TCR repertoires between the LTNP group and the other HIV-infected individuals. However, there was a statistically significant inverse correlation between CD4 count and the number of Vβ family-specific perturbations in the recent seroconverters (SC) and those with progressive infection (PI), consistent with other shared features of clinical disease progression (Th1/Th2 switch and high frequency of vital isolation). We conclude that long-term clinical nonprogression in HIV-1 infection is not associated with the loss or expansion of a particular Vβ family; instead low CD4 count in the PI and SC individuals was correlated with increased number of Vβ family-specific perturbations relative to the LTNP group and that it is hence unlikely that HIV encodes a superantigen.

Original languageEnglish
Pages (from-to)1187-1196
Number of pages10
JournalBritish Journal of Haematology
Volume102
Issue number5
DOIs
Publication statusPublished - 1998

    Fingerprint

Keywords

  • Anchor-PCR
  • Disease progression
  • HIV
  • TCR
  • V-beta repertoire

ASJC Scopus subject areas

  • Hematology

Cite this