Low-Cost Fluorescein Detection System for High-Grade Glioma Surgery

Daniele Bongetta, Cesare Zoia, Raffaelino Pugliese, Daniela Adinolfi, Vittorio Silvani, Paolo Gaetani

Research output: Contribution to journalArticlepeer-review


Background Intraoperative fluorescein detection has been used in the fields of vascular and oncologic neurosurgery since 1948. Modifications of the optics in order to enhance the fluorescence contrast under microscopic view have been developed by many authors. The industries, during the past 10 years, provided commercial high-cost optimized apparatuses. Reviewing the literature, we found that the prototypical techniques were definitely inexpensive but lacked reliability, reproducibility, and standard legal norms. Methods We describe the developing of a fluorescein detection system that could be economic, simple, effective, and law abiding. Results We employed a commercial violet-blue filter designed for fluorescein excitation in endoscopic procedures and used commercial photographic yellow optical filters for fluorescence detection. All the instrumentation is cleared for clinical use, and its cost is up to 200 times lower than commercial apparatuses. Conclusion Our results show a good distinction of fluorescein-stained structures, with overall acceptable operating light conditions.

Original languageEnglish
Pages (from-to)54-58
Number of pages5
JournalWorld Neurosurgery
Publication statusPublished - Apr 1 2016


  • Filter
  • Fluorescein
  • Glioma
  • Key words Endoscopy
  • Low-cost

ASJC Scopus subject areas

  • Clinical Neurology
  • Surgery


Dive into the research topics of 'Low-Cost Fluorescein Detection System for High-Grade Glioma Surgery'. Together they form a unique fingerprint.

Cite this