Low density lipoprotein receptor and mRNA expression in human colorectal cancer

M. G. Caruso, M. Notarnicola, A. Cavallini, A. Di Leo

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: We have previously show that, 63.3% of colorectal cancers did not express the Low Density Lipoprotein Receptor (LDLR). We now report the findings of a study on the expression of LDLR and its mRNA in neoplastic tissue specimens of human colorectal cancer (CRC), carried out to verify whether the absence of the LDLR in these turnouts is reflected by the absence of its transcript. Materials and Methods: 32 patients (10 females and 22 males) operated for CRC were included in the study. The LDLR levels were evaluated by immunoenzymatic assay. The LDLR-mRNA, reverse-transcribed and then amplified by polymerase chain reaction, was detected by chromatography. Results: The LDLR protein was present in 12 out of the 32 patients. LDLR-mRNA expression was detected in 17 out of the 32 patients. The absence of the LDLR protein was reflected by the absence of its transcript in 13 out of the 20 tumours; The LDLR mRNA levels were significantly higher in the tumours that did not express LDLR. Conclusion: The variable expression of the LDLR protein and LDLR mRNA in CRC detected in this study suggests the possibility that different therapeutic strategies may be indicated for these tumours.

Original languageEnglish
Pages (from-to)429-434
Number of pages6
JournalAnticancer Research
Volume21
Issue number1 A
Publication statusPublished - 2001

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LDL Receptors
Colorectal Neoplasms
Messenger RNA
Neoplasms
Proteins
Chromatography

Keywords

  • Colorectal cancer
  • LDL receptor
  • mRNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Low density lipoprotein receptor and mRNA expression in human colorectal cancer. / Caruso, M. G.; Notarnicola, M.; Cavallini, A.; Di Leo, A.

In: Anticancer Research, Vol. 21, No. 1 A, 2001, p. 429-434.

Research output: Contribution to journalArticle

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AB - Background: We have previously show that, 63.3% of colorectal cancers did not express the Low Density Lipoprotein Receptor (LDLR). We now report the findings of a study on the expression of LDLR and its mRNA in neoplastic tissue specimens of human colorectal cancer (CRC), carried out to verify whether the absence of the LDLR in these turnouts is reflected by the absence of its transcript. Materials and Methods: 32 patients (10 females and 22 males) operated for CRC were included in the study. The LDLR levels were evaluated by immunoenzymatic assay. The LDLR-mRNA, reverse-transcribed and then amplified by polymerase chain reaction, was detected by chromatography. Results: The LDLR protein was present in 12 out of the 32 patients. LDLR-mRNA expression was detected in 17 out of the 32 patients. The absence of the LDLR protein was reflected by the absence of its transcript in 13 out of the 20 tumours; The LDLR mRNA levels were significantly higher in the tumours that did not express LDLR. Conclusion: The variable expression of the LDLR protein and LDLR mRNA in CRC detected in this study suggests the possibility that different therapeutic strategies may be indicated for these tumours.

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