Low effectiveness of DNA vaccination against HER-2/neu in ageing

Mauro Provinciali, Arianna Smorlesi, Alessia Donnini, Beatrice Bartozzi, Augusto Amici

Research output: Contribution to journalArticlepeer-review

Abstract

We evaluated the effectiveness of vaccination with a HER-2/neu DNA plasmid to induce protective immunity against HER-2/neu overexpressing syngeneic TUBO tumour cells in old ages. Young and old Balb/c mice received three immunizations with a pCMVneuNT DNA plasmid and, successively, were challenged with TUBO cells. Young mice were completely protected whereas less than 60% protection was observed in old mice. Anti-p185neu antibodies were found in the sera from both young and old immunized mice, even if antibody production was significantly higher in young in comparison with old mice. Similarly, higher anti-p185neu lymphocyte proliferation was induced in young than in old mice. No anti-p185neu cytotoxicity was found in lymphocytes from old animals. We conclude that anticancer DNA vaccination has a lower effectiveness in old than in young ages.

Original languageEnglish
Pages (from-to)843-848
Number of pages6
JournalVaccine
Volume21
Issue number9-10
DOIs
Publication statusPublished - Feb 14 2003

Keywords

  • Ageing
  • DNA vaccination
  • HER-2/neu

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

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