Low hepcidin accounts for the proinflammatory status associated with iron deficiency

Alessia Pagani, Antonella Nai, Gianfranca Corna, Lidia Bosurgi, Patrizia Rovere-Querini, Clara Camaschella, Laura Silvestri

Research output: Contribution to journalArticle

Abstract

Hepcidin is an antimicrobial peptide that controls systemic iron homeostasis. Hepcidin binding to its receptor ferroportin reduces iron availability, thus controlling microbial growth. In parallel it triggers an anti-inflammatory response in macrophages. Hepcidin is transcriptionally regulated by iron, through the bone morphogenetic protein-son of mothers against decapentaplegic (BMP-SMAD) pathway and by inflammation, through IL6-mediated STAT3 signaling. To investigate the mechanisms linking iron and inflammation, we treated C57BL/6 iron-deficient mice with a sublethal dose of lipopolysaccharide (LPS) and analyzed their inflammatory response in comparison with controls. We show that iron-deprived mice have a proinflammatory condition, exacerbated by LPS treatment leading to increased IL6 and TNFα mRNA in liver and spleen macrophages, and increased serum IL6 (482.29 ± 205.59 pg/mL) versus controls (69.01 ± 17.52 pg/mL; P <.05). Hepcidin was undetectable in iron-deficient mice but pretreatment with hepcidin normalized their response to LPS. Tmprss6-/- mice, characterized by iron deficiency and high hepcidin, show a blunted inflammatory response when challenged with LPS. Our data support a model in which the lack of hepcidin is responsible of the high inflammatory response to LPS in iron deficiency. The proinflammatory status associated with chronic iron deficiency could explain the resistance to infection seen in this condition.

Original languageEnglish
Pages (from-to)736-746
Number of pages11
JournalBlood
Volume118
Issue number3
DOIs
Publication statusPublished - Jul 21 2011

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Hepcidins
Iron
Lipopolysaccharides
Interleukin-6
Macrophages
Inflammation
Bone Morphogenetic Proteins
Liver
Homeostasis
Anti-Inflammatory Agents
Spleen
Availability

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology

Cite this

Pagani, A., Nai, A., Corna, G., Bosurgi, L., Rovere-Querini, P., Camaschella, C., & Silvestri, L. (2011). Low hepcidin accounts for the proinflammatory status associated with iron deficiency. Blood, 118(3), 736-746. https://doi.org/10.1182/blood-2011-02-337212

Low hepcidin accounts for the proinflammatory status associated with iron deficiency. / Pagani, Alessia; Nai, Antonella; Corna, Gianfranca; Bosurgi, Lidia; Rovere-Querini, Patrizia; Camaschella, Clara; Silvestri, Laura.

In: Blood, Vol. 118, No. 3, 21.07.2011, p. 736-746.

Research output: Contribution to journalArticle

Pagani, A, Nai, A, Corna, G, Bosurgi, L, Rovere-Querini, P, Camaschella, C & Silvestri, L 2011, 'Low hepcidin accounts for the proinflammatory status associated with iron deficiency', Blood, vol. 118, no. 3, pp. 736-746. https://doi.org/10.1182/blood-2011-02-337212
Pagani, Alessia ; Nai, Antonella ; Corna, Gianfranca ; Bosurgi, Lidia ; Rovere-Querini, Patrizia ; Camaschella, Clara ; Silvestri, Laura. / Low hepcidin accounts for the proinflammatory status associated with iron deficiency. In: Blood. 2011 ; Vol. 118, No. 3. pp. 736-746.
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