Low molecular mass proteins and urinary enzymes in amniotic fluid of healthy pregnant women at progressive stages of gestation

Michele Mussap, Vassilios Fanos, Antonio Piccoli, Martina Zaninotto, Ezio M. Padovani, Mario Plebani

Research output: Contribution to journalArticlepeer-review

Abstract

Objectives: Amniotic fluid α1-microglobulin (α1-m) and β2-microglobulin (β2-m) levels, as well as N-acetyl-β-D-glucosaminidase (NAG) and alanine aminopeptidase (AAP) activities, were measured in the course of uncomplicated pregnancies to assess their variations as gestation progresses. Design and Methods: Samples were obtained from 141 healthy pregnant women divided into three groups on the basis of gestational stage. Quantitative estimation of proteins was performed immunometrically and enzyme activities were determined spectrophotometrically. Results: It was found that, during pregnancy, α1-m and β2-m concentrations as well as AAP activity significantly decrease, although their reduction patterns vary. Controversial results were found for NAG activity: the normalization of values for amniotic fluid creatinine significantly changed the reduction pattern of this enzyme. No statistically significant differences were found between male and female fetuses for amniotic fluid values of the biochemical substances studied. Conclusions: The behavior observed for α1m, β2-m, and AAP might be linked to the progressive development and maturation of fetal renal tubular function. Amniotic fluid total NAG activity seems not to depend only on fetal urinary excretion.

Original languageEnglish
Pages (from-to)51-55
Number of pages5
JournalClinical Biochemistry
Volume29
Issue number1
DOIs
Publication statusPublished - Feb 1996

Keywords

  • α-microglobulin
  • β-microglobulin
  • Alanine aminopeptidase
  • Amniotic fluid
  • N-acetyl-β-D-glucosaminidase

ASJC Scopus subject areas

  • Clinical Biochemistry

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