Low prevalence of HPV-driven head and neck squamous cell carcinoma in North-East Italy

Lorena Baboci, Dana Holzinger, Paolo Boscolo-Rizzo, Giancarlo Tirelli, Roberto Spinato, Valentina Lupato, Roberto Fuson, Markus Schmitt, Angelika Michel, Gordana Halec, Maria Cristina Da Mosto, Michael Pawlita, Annarosa Del Mistro

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Objectives To investigate the frequency of Human papillomavirus (HPV)-driven head and neck squamous cell carcinoma (HNSCC) among patients living in North-East Italy, by assessing HPV-DNA positivity in all tumors and additional markers whenever possible. Material and methods HPV types, viral load, viral RNA, HPV16/18 E6 protein and p16INK4a and pRb expression were determined in primary tumor tissues from 247 HNSCC patients. Tumor-specific HPV seropositivity was analyzed in 102 patients. Results Tumor HPV-DNA prevalence was 8.5% overall (21/247) and 27% in oropharynx (17/63). HPV16 accounted for 95% of all HPV types found. Among HPV-DNA+ tumors, type-concordant HPV E6*I RNA prevalence was 79%. HPV DNA+ RNA+ tumors showed high viral load, up-regulated p16INK4a, down-regulated pRb and presence of HPV16 E6 protein. Eight cases showed tumor-specific HPV seropositivity, all type-concordant with the tumor. Tumors were defined as HPV-driven when positive for HPV-DNA plus 2 additional HPV transformation-related markers. Conclusion Relative prevalence of HPV-driven tumors (14 HPV16, 1 HPV58) was 6% overall and 20% among oropharyngeal cancers. In the oropharynx the HPV-driven group showed a trend for better survival versus the HPV-negative group. The relative prevalence of HPV-driven oropharyngeal cancer is low in North-East Italy as compared to Western and Northern Europe.

Original languageEnglish
Pages (from-to)133-140
Number of pages8
JournalPapillomavirus Research
Volume2
DOIs
Publication statusPublished - Dec 1 2016

Fingerprint

Italy
Neoplasms
DNA
Oropharyngeal Neoplasms
Carcinoma, squamous cell of head and neck
Oropharynx
Viral Load
Cyclin-Dependent Kinase Inhibitor p16
Viral RNA
Tumor Biomarkers

Keywords

  • Head and neck cancer
  • HPV antibodies
  • HPV-driven
  • HPV-related markers
  • Oropharyngeal cancer
  • Prevalence

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Low prevalence of HPV-driven head and neck squamous cell carcinoma in North-East Italy. / Baboci, Lorena; Holzinger, Dana; Boscolo-Rizzo, Paolo; Tirelli, Giancarlo; Spinato, Roberto; Lupato, Valentina; Fuson, Roberto; Schmitt, Markus; Michel, Angelika; Halec, Gordana; Da Mosto, Maria Cristina; Pawlita, Michael; Del Mistro, Annarosa.

In: Papillomavirus Research, Vol. 2, 01.12.2016, p. 133-140.

Research output: Contribution to journalArticle

Baboci, L, Holzinger, D, Boscolo-Rizzo, P, Tirelli, G, Spinato, R, Lupato, V, Fuson, R, Schmitt, M, Michel, A, Halec, G, Da Mosto, MC, Pawlita, M & Del Mistro, A 2016, 'Low prevalence of HPV-driven head and neck squamous cell carcinoma in North-East Italy', Papillomavirus Research, vol. 2, pp. 133-140. https://doi.org/10.1016/j.pvr.2016.07.002
Baboci L, Holzinger D, Boscolo-Rizzo P, Tirelli G, Spinato R, Lupato V et al. Low prevalence of HPV-driven head and neck squamous cell carcinoma in North-East Italy. Papillomavirus Research. 2016 Dec 1;2:133-140. https://doi.org/10.1016/j.pvr.2016.07.002
Baboci, Lorena ; Holzinger, Dana ; Boscolo-Rizzo, Paolo ; Tirelli, Giancarlo ; Spinato, Roberto ; Lupato, Valentina ; Fuson, Roberto ; Schmitt, Markus ; Michel, Angelika ; Halec, Gordana ; Da Mosto, Maria Cristina ; Pawlita, Michael ; Del Mistro, Annarosa. / Low prevalence of HPV-driven head and neck squamous cell carcinoma in North-East Italy. In: Papillomavirus Research. 2016 ; Vol. 2. pp. 133-140.
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abstract = "Objectives To investigate the frequency of Human papillomavirus (HPV)-driven head and neck squamous cell carcinoma (HNSCC) among patients living in North-East Italy, by assessing HPV-DNA positivity in all tumors and additional markers whenever possible. Material and methods HPV types, viral load, viral RNA, HPV16/18 E6 protein and p16INK4a and pRb expression were determined in primary tumor tissues from 247 HNSCC patients. Tumor-specific HPV seropositivity was analyzed in 102 patients. Results Tumor HPV-DNA prevalence was 8.5{\%} overall (21/247) and 27{\%} in oropharynx (17/63). HPV16 accounted for 95{\%} of all HPV types found. Among HPV-DNA+ tumors, type-concordant HPV E6*I RNA prevalence was 79{\%}. HPV DNA+ RNA+ tumors showed high viral load, up-regulated p16INK4a, down-regulated pRb and presence of HPV16 E6 protein. Eight cases showed tumor-specific HPV seropositivity, all type-concordant with the tumor. Tumors were defined as HPV-driven when positive for HPV-DNA plus 2 additional HPV transformation-related markers. Conclusion Relative prevalence of HPV-driven tumors (14 HPV16, 1 HPV58) was 6{\%} overall and 20{\%} among oropharyngeal cancers. In the oropharynx the HPV-driven group showed a trend for better survival versus the HPV-negative group. The relative prevalence of HPV-driven oropharyngeal cancer is low in North-East Italy as compared to Western and Northern Europe.",
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author = "Lorena Baboci and Dana Holzinger and Paolo Boscolo-Rizzo and Giancarlo Tirelli and Roberto Spinato and Valentina Lupato and Roberto Fuson and Markus Schmitt and Angelika Michel and Gordana Halec and {Da Mosto}, {Maria Cristina} and Michael Pawlita and {Del Mistro}, Annarosa",
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AU - Spinato, Roberto

AU - Lupato, Valentina

AU - Fuson, Roberto

AU - Schmitt, Markus

AU - Michel, Angelika

AU - Halec, Gordana

AU - Da Mosto, Maria Cristina

AU - Pawlita, Michael

AU - Del Mistro, Annarosa

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N2 - Objectives To investigate the frequency of Human papillomavirus (HPV)-driven head and neck squamous cell carcinoma (HNSCC) among patients living in North-East Italy, by assessing HPV-DNA positivity in all tumors and additional markers whenever possible. Material and methods HPV types, viral load, viral RNA, HPV16/18 E6 protein and p16INK4a and pRb expression were determined in primary tumor tissues from 247 HNSCC patients. Tumor-specific HPV seropositivity was analyzed in 102 patients. Results Tumor HPV-DNA prevalence was 8.5% overall (21/247) and 27% in oropharynx (17/63). HPV16 accounted for 95% of all HPV types found. Among HPV-DNA+ tumors, type-concordant HPV E6*I RNA prevalence was 79%. HPV DNA+ RNA+ tumors showed high viral load, up-regulated p16INK4a, down-regulated pRb and presence of HPV16 E6 protein. Eight cases showed tumor-specific HPV seropositivity, all type-concordant with the tumor. Tumors were defined as HPV-driven when positive for HPV-DNA plus 2 additional HPV transformation-related markers. Conclusion Relative prevalence of HPV-driven tumors (14 HPV16, 1 HPV58) was 6% overall and 20% among oropharyngeal cancers. In the oropharynx the HPV-driven group showed a trend for better survival versus the HPV-negative group. The relative prevalence of HPV-driven oropharyngeal cancer is low in North-East Italy as compared to Western and Northern Europe.

AB - Objectives To investigate the frequency of Human papillomavirus (HPV)-driven head and neck squamous cell carcinoma (HNSCC) among patients living in North-East Italy, by assessing HPV-DNA positivity in all tumors and additional markers whenever possible. Material and methods HPV types, viral load, viral RNA, HPV16/18 E6 protein and p16INK4a and pRb expression were determined in primary tumor tissues from 247 HNSCC patients. Tumor-specific HPV seropositivity was analyzed in 102 patients. Results Tumor HPV-DNA prevalence was 8.5% overall (21/247) and 27% in oropharynx (17/63). HPV16 accounted for 95% of all HPV types found. Among HPV-DNA+ tumors, type-concordant HPV E6*I RNA prevalence was 79%. HPV DNA+ RNA+ tumors showed high viral load, up-regulated p16INK4a, down-regulated pRb and presence of HPV16 E6 protein. Eight cases showed tumor-specific HPV seropositivity, all type-concordant with the tumor. Tumors were defined as HPV-driven when positive for HPV-DNA plus 2 additional HPV transformation-related markers. Conclusion Relative prevalence of HPV-driven tumors (14 HPV16, 1 HPV58) was 6% overall and 20% among oropharyngeal cancers. In the oropharynx the HPV-driven group showed a trend for better survival versus the HPV-negative group. The relative prevalence of HPV-driven oropharyngeal cancer is low in North-East Italy as compared to Western and Northern Europe.

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