Low red blood cell levels of deglycating enzymes in colorectal cancer patients

Maria Notarnicola, Maria Gabriella Caruso, Valeria Tutino, Vito Guerra, Giovanni Misciagna

Research output: Contribution to journalArticle

Abstract

AIM: To investigate Glyoxalase I and fructosamine-3-kinase (FN3K) activity in red blood cells from patients with colorectal adenomas and cancer. METHODS: Thirty three consecutive subjects with one or more histologically confirmed colorectal adenomatous polyps, 16 colorectal cancer patients and a group of 11 control subjects with normal colonoscopy were included in the study. Glyoxalase I and FN3K activities were measured in red blood cells using a spectrophotometric and radiometric assay, respectively. RESULTS: A significant reduction in both Glyoxalase I and FN3K activity was detected in patients with tumors compared to patients with adenomas and the controls. Erythrocyte Glyoxalase I activity in colorectal cancer was approximately 6 times lower than that detected in patients with adenoma (0.022 ± 0.01 mmol/min per milliliter vs 0.128 ± 0.19 mmol/min per milliliter of red blood cells, P = 0.003, Tukey's test). FN3K activity in red blood cells from patients with colon cancer was approximately 2 times lower than that detected in adenoma patients (19.55 ± 6.4 pmol/min per milliliter vs 38.6 ± 31.7 pmol/min per milliliter of red blood cells, P = 0.04, Tukey's test). CONCLUSION: These findings suggest that deglycating enzymes may be involved in the malignant transformation of colon mucosa.

Original languageEnglish
Pages (from-to)329-333
Number of pages5
JournalWorld Journal of Gastroenterology
Volume17
Issue number3
DOIs
Publication statusPublished - Jan 21 2011

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Keywords

  • Colorectal cancer
  • Enzymatic activity
  • Fruc-tosamine-3-kinase
  • Glycation
  • Glyoxalase I

ASJC Scopus subject areas

  • Gastroenterology

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