Lower prevalence of Blastocystis sp. infections in HIV positive compared to HIV negative adults in Ghana

Veronica Di Cristanziano, Rossella D'Alfonso, Federica Berrilli, Fred Stephen Sarfo, Maristella Santoro, Lavinia Fabeni, Elena Knops, Eva Heger, Rolf Kaiser, Albert Dompreh, Richard Odame Phillips, Betty Norman, Torsten Feldt, Kirsten Alexandra Eberhardt

Research output: Contribution to journalArticle

Abstract

Background Sub-Saharan Africa is endemic for intestinal parasites and distinguished for the largest burden of HIV cases. Blastocystis sp. is one of the most common protists infecting humans but its role in human disease is still controversial. Aim of this study was to investigate the prevalence of Blastocystis sp. in HIV positive and negative adults in Ghana and its association with immune status and other risk factors. Methods 122 HIV positive outpatients and 70 HIV negative blood donors from the Komfo Anokye Teaching Hospital in Kumasi, Ghana, were included in the present study. Demographic, clinical and laboratory data were collected and HIV positive patients distinguished for CD4+ T cell count <200 cells/μl (n = 54) and >200 cells/μl (n = 68). A Blastocystis's phylogenetic analysis was performed to determine sample subtype (ST). Results The prevalence of Blastocystis sp. in adult HIV positive individuals was lower than in HIV negative persons (6.6% vs. 20.0%, p = 0.008) and Blastocystis sp. ST1 was the most prevalent strain. Within HIV positive participants, the prevalence of Blastocystis sp. was lower in those individuals with CD4+ T cell count <200 cells/μl than in patients with higher CD4+ T cell count (1.9% vs. 10.3%, p = 0.076). Multiple regression analysis revealed that Blastocystis sp. was inversely associated with an obese Body Mass Index (BMI) in HIV negative persons (p = 0.040). Presence of Blastocystis sp. was correlated with higher CD4+ T cell count in HIV positive participants (p = 0.049). Conclusion It is largely reported that people living with HIV (PLHIV) in Africa are affected from parasite infections and that co-infections may adversely impact on their immune status, accelerating progress to AIDS and worsening gastrointestinal manifestations. Differently, in this study Blastocystis sp. was associated with a better immune status jointly with a healthy body weight while it seems to be reduced with the progression of HIV infection. This data agree with recent suggestions that Blastocystis sp. can represent a component of the healthy gut microbiota.

Original languageEnglish
Article numbere0221968
Pages (from-to)1-15
Number of pages15
JournalPLoS One
Volume14
Issue number9
DOIs
Publication statusPublished - Jan 1 2019

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Blastocystis
Ghana
T-cells
HIV infections
HIV Infections
HIV
CD4 Lymphocyte Count
T-lymphocytes
T-Lymphocytes
Regression analysis
Teaching
Blood
parasites
Parasitic Diseases
Sub-Saharan Africa
Africa South of the Sahara
mixed infection
intestinal microorganisms
human diseases
Blood Donors

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Cristanziano, V. D., D'Alfonso, R., Berrilli, F., Sarfo, F. S., Santoro, M., Fabeni, L., ... Eberhardt, K. A. (2019). Lower prevalence of Blastocystis sp. infections in HIV positive compared to HIV negative adults in Ghana. PLoS One, 14(9), 1-15. [e0221968]. https://doi.org/10.1371/journal.pone.0221968

Lower prevalence of Blastocystis sp. infections in HIV positive compared to HIV negative adults in Ghana. / Cristanziano, Veronica Di; D'Alfonso, Rossella; Berrilli, Federica; Sarfo, Fred Stephen; Santoro, Maristella; Fabeni, Lavinia; Knops, Elena; Heger, Eva; Kaiser, Rolf; Dompreh, Albert; Phillips, Richard Odame; Norman, Betty; Feldt, Torsten; Eberhardt, Kirsten Alexandra.

In: PLoS One, Vol. 14, No. 9, e0221968, 01.01.2019, p. 1-15.

Research output: Contribution to journalArticle

Cristanziano, VD, D'Alfonso, R, Berrilli, F, Sarfo, FS, Santoro, M, Fabeni, L, Knops, E, Heger, E, Kaiser, R, Dompreh, A, Phillips, RO, Norman, B, Feldt, T & Eberhardt, KA 2019, 'Lower prevalence of Blastocystis sp. infections in HIV positive compared to HIV negative adults in Ghana', PLoS One, vol. 14, no. 9, e0221968, pp. 1-15. https://doi.org/10.1371/journal.pone.0221968
Cristanziano VD, D'Alfonso R, Berrilli F, Sarfo FS, Santoro M, Fabeni L et al. Lower prevalence of Blastocystis sp. infections in HIV positive compared to HIV negative adults in Ghana. PLoS One. 2019 Jan 1;14(9):1-15. e0221968. https://doi.org/10.1371/journal.pone.0221968
Cristanziano, Veronica Di ; D'Alfonso, Rossella ; Berrilli, Federica ; Sarfo, Fred Stephen ; Santoro, Maristella ; Fabeni, Lavinia ; Knops, Elena ; Heger, Eva ; Kaiser, Rolf ; Dompreh, Albert ; Phillips, Richard Odame ; Norman, Betty ; Feldt, Torsten ; Eberhardt, Kirsten Alexandra. / Lower prevalence of Blastocystis sp. infections in HIV positive compared to HIV negative adults in Ghana. In: PLoS One. 2019 ; Vol. 14, No. 9. pp. 1-15.
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abstract = "Background Sub-Saharan Africa is endemic for intestinal parasites and distinguished for the largest burden of HIV cases. Blastocystis sp. is one of the most common protists infecting humans but its role in human disease is still controversial. Aim of this study was to investigate the prevalence of Blastocystis sp. in HIV positive and negative adults in Ghana and its association with immune status and other risk factors. Methods 122 HIV positive outpatients and 70 HIV negative blood donors from the Komfo Anokye Teaching Hospital in Kumasi, Ghana, were included in the present study. Demographic, clinical and laboratory data were collected and HIV positive patients distinguished for CD4+ T cell count <200 cells/μl (n = 54) and >200 cells/μl (n = 68). A Blastocystis's phylogenetic analysis was performed to determine sample subtype (ST). Results The prevalence of Blastocystis sp. in adult HIV positive individuals was lower than in HIV negative persons (6.6{\%} vs. 20.0{\%}, p = 0.008) and Blastocystis sp. ST1 was the most prevalent strain. Within HIV positive participants, the prevalence of Blastocystis sp. was lower in those individuals with CD4+ T cell count <200 cells/μl than in patients with higher CD4+ T cell count (1.9{\%} vs. 10.3{\%}, p = 0.076). Multiple regression analysis revealed that Blastocystis sp. was inversely associated with an obese Body Mass Index (BMI) in HIV negative persons (p = 0.040). Presence of Blastocystis sp. was correlated with higher CD4+ T cell count in HIV positive participants (p = 0.049). Conclusion It is largely reported that people living with HIV (PLHIV) in Africa are affected from parasite infections and that co-infections may adversely impact on their immune status, accelerating progress to AIDS and worsening gastrointestinal manifestations. Differently, in this study Blastocystis sp. was associated with a better immune status jointly with a healthy body weight while it seems to be reduced with the progression of HIV infection. This data agree with recent suggestions that Blastocystis sp. can represent a component of the healthy gut microbiota.",
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AU - Sarfo, Fred Stephen

AU - Santoro, Maristella

AU - Fabeni, Lavinia

AU - Knops, Elena

AU - Heger, Eva

AU - Kaiser, Rolf

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AU - Phillips, Richard Odame

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AU - Eberhardt, Kirsten Alexandra

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N2 - Background Sub-Saharan Africa is endemic for intestinal parasites and distinguished for the largest burden of HIV cases. Blastocystis sp. is one of the most common protists infecting humans but its role in human disease is still controversial. Aim of this study was to investigate the prevalence of Blastocystis sp. in HIV positive and negative adults in Ghana and its association with immune status and other risk factors. Methods 122 HIV positive outpatients and 70 HIV negative blood donors from the Komfo Anokye Teaching Hospital in Kumasi, Ghana, were included in the present study. Demographic, clinical and laboratory data were collected and HIV positive patients distinguished for CD4+ T cell count <200 cells/μl (n = 54) and >200 cells/μl (n = 68). A Blastocystis's phylogenetic analysis was performed to determine sample subtype (ST). Results The prevalence of Blastocystis sp. in adult HIV positive individuals was lower than in HIV negative persons (6.6% vs. 20.0%, p = 0.008) and Blastocystis sp. ST1 was the most prevalent strain. Within HIV positive participants, the prevalence of Blastocystis sp. was lower in those individuals with CD4+ T cell count <200 cells/μl than in patients with higher CD4+ T cell count (1.9% vs. 10.3%, p = 0.076). Multiple regression analysis revealed that Blastocystis sp. was inversely associated with an obese Body Mass Index (BMI) in HIV negative persons (p = 0.040). Presence of Blastocystis sp. was correlated with higher CD4+ T cell count in HIV positive participants (p = 0.049). Conclusion It is largely reported that people living with HIV (PLHIV) in Africa are affected from parasite infections and that co-infections may adversely impact on their immune status, accelerating progress to AIDS and worsening gastrointestinal manifestations. Differently, in this study Blastocystis sp. was associated with a better immune status jointly with a healthy body weight while it seems to be reduced with the progression of HIV infection. This data agree with recent suggestions that Blastocystis sp. can represent a component of the healthy gut microbiota.

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