Lowering of circulating free-fatty acids levels and reduced expression of leptin in white adipose tissue in postobesity status

Aldo V. Greco, Geltrude Mingrone, Roberto Vettor, Melania Manco, Giuseppina Rosa, Esmeralda Capristo, Giovanni Federspil, Marco Castagneto, Giovanni Gasbarrini

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Abstract

Background: Our aim was to investigate the regulation of the gene expression of leptin in subcutaneous adipose tissue biopsies in morbid obesity before and after biliopancreatic diversion (BPD). Methods: Longitudinal study in morbidly obese subjects investigated twice: before and 6 months after BPD. Fourteen morbidly obese women, 37±13 years old and with a body mass index of 51.6±8.2 kg/m2, were studied before and 6 months after BPD (40.6±8.0 kg/m2). Using reverse transcriptase polymerase chain reaction analysis, the mRNA expression of leptin was investigated in adipose tissue. Plasma leptin was measured by radioimmunoassay; plasma insulin was measured by microparticle enzyme immunoassay. Free fatty acids (FFA) were measured using a colorimetric kit. Results: A significant decrease in leptin mRNA level was observed in comparison with pretreatment in BPD patients (59±34 vs 143±85 arbitrary units, P2=0.80, P2=0.86, P2=0.65, P2=0.92, P2=0.64, P2=0.88, P2=0.83, P

Original languageEnglish
Pages (from-to)207-213
Number of pages7
JournalJournal of Investigative Medicine
Volume50
Issue number3
Publication statusPublished - 2002

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Keywords

  • Biliopancreatic diversion
  • Free-fatty acids
  • Leptin
  • Morbid obesity

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Greco, A. V., Mingrone, G., Vettor, R., Manco, M., Rosa, G., Capristo, E., Federspil, G., Castagneto, M., & Gasbarrini, G. (2002). Lowering of circulating free-fatty acids levels and reduced expression of leptin in white adipose tissue in postobesity status. Journal of Investigative Medicine, 50(3), 207-213.