TY - JOUR
T1 - Lowering of circulating free-fatty acids levels and reduced expression of leptin in white adipose tissue in postobesity status
AU - Greco, Aldo V.
AU - Mingrone, Geltrude
AU - Vettor, Roberto
AU - Manco, Melania
AU - Rosa, Giuseppina
AU - Capristo, Esmeralda
AU - Federspil, Giovanni
AU - Castagneto, Marco
AU - Gasbarrini, Giovanni
PY - 2002
Y1 - 2002
N2 - Background: Our aim was to investigate the regulation of the gene expression of leptin in subcutaneous adipose tissue biopsies in morbid obesity before and after biliopancreatic diversion (BPD). Methods: Longitudinal study in morbidly obese subjects investigated twice: before and 6 months after BPD. Fourteen morbidly obese women, 37±13 years old and with a body mass index of 51.6±8.2 kg/m2, were studied before and 6 months after BPD (40.6±8.0 kg/m2). Using reverse transcriptase polymerase chain reaction analysis, the mRNA expression of leptin was investigated in adipose tissue. Plasma leptin was measured by radioimmunoassay; plasma insulin was measured by microparticle enzyme immunoassay. Free fatty acids (FFA) were measured using a colorimetric kit. Results: A significant decrease in leptin mRNA level was observed in comparison with pretreatment in BPD patients (59±34 vs 143±85 arbitrary units, P2=0.80, P2=0.86, P2=0.65, P2=0.92, P2=0.64, P2=0.88, P2=0.83, P
AB - Background: Our aim was to investigate the regulation of the gene expression of leptin in subcutaneous adipose tissue biopsies in morbid obesity before and after biliopancreatic diversion (BPD). Methods: Longitudinal study in morbidly obese subjects investigated twice: before and 6 months after BPD. Fourteen morbidly obese women, 37±13 years old and with a body mass index of 51.6±8.2 kg/m2, were studied before and 6 months after BPD (40.6±8.0 kg/m2). Using reverse transcriptase polymerase chain reaction analysis, the mRNA expression of leptin was investigated in adipose tissue. Plasma leptin was measured by radioimmunoassay; plasma insulin was measured by microparticle enzyme immunoassay. Free fatty acids (FFA) were measured using a colorimetric kit. Results: A significant decrease in leptin mRNA level was observed in comparison with pretreatment in BPD patients (59±34 vs 143±85 arbitrary units, P2=0.80, P2=0.86, P2=0.65, P2=0.92, P2=0.64, P2=0.88, P2=0.83, P
KW - Biliopancreatic diversion
KW - Free-fatty acids
KW - Leptin
KW - Morbid obesity
UR - http://www.scopus.com/inward/record.url?scp=0036097248&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036097248&partnerID=8YFLogxK
M3 - Article
C2 - 12033286
AN - SCOPUS:0036097248
VL - 50
SP - 207
EP - 213
JO - Journal of Investigative Medicine
JF - Journal of Investigative Medicine
SN - 1081-5589
IS - 3
ER -