LPS-inducible nuclear factor in human monocytes that binds the negative regulatory element of the HIV LTR

T. Musso, M. C. Bosco, D. L. Longo, I. Espinoza-Delgado, A. Sica, G. W. Cox, G. L. Gusella, G. Forni, L. Varesio

Research output: Contribution to journalArticle

Abstract

We studied the constitutive and lipopolysaccharide (LPS)-induced expression of nuclear protein binding to the negative regulatory element (NRE) of the human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) in fresh human monocytes. We demonstrated the existence of a constitutive factor binding to the NRE 73-bp HpaII/HpaII fragment (-216 to -143) whose expression is up-regulated by LPS treatment. Competition experiments with overlapping oligonucleotides covering the NpaII/HpaII fragment and with mutated oligonucleotides mapped the binding within the TTTCATCAC region (-171 to -163). This binding pattern is unique to human monocytes.

Original languageEnglish
Pages (from-to)21-26
Number of pages6
JournalJournal of Leukocyte Biology
Volume56
Issue number1
Publication statusPublished - 1994

Keywords

  • HIV
  • Human monocytes
  • Lipopolysaccharide
  • Nuclear factor

ASJC Scopus subject areas

  • Cell Biology

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