LSD1 regulates the balance between self-renewal and differentiation in human embryonic stem cells

Antonio Adamo, Borja Sesé, Stephanie Boue, Julio Castaño, Ida Paramonov, Maria J. Barrero, Juan Carlos Izpisua Belmonte

Research output: Contribution to journalArticle

Abstract

We identify LSD1 (lysine-specific demethylase 1; also known as KDM1A and AOF2) as a key histone modifier that participates in the maintenance of pluripotency through the regulation of bivalent domains, a chromatin environment present at the regulatory regions of developmental genes that contains both H3K4 di/trimethylation and H3K27 trimethylation marks. LSD1 occupies the promoters of a subset of developmental genes that contain bivalent domains and are co-occupied by OCT4 and NANOG in human embryonic stem cells, where it controls the levels of H3K4 methylation through its demethylase activity. Thus, LSD1 has a role in maintaining the silencing of several developmental genes in human embryonic stem cells by regulating the critical balance between H3K4 and H3K27 methylation at their regulatory regions.

Original languageEnglish
Pages (from-to)652-661
Number of pages10
JournalNature Cell Biology
Volume13
Issue number6
DOIs
Publication statusPublished - Jun 2011

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Developmental Genes
Nucleic Acid Regulatory Sequences
Methylation
Histones
Lysine
Chromatin
Maintenance
Human Embryonic Stem Cells

ASJC Scopus subject areas

  • Cell Biology

Cite this

Adamo, A., Sesé, B., Boue, S., Castaño, J., Paramonov, I., Barrero, M. J., & Belmonte, J. C. I. (2011). LSD1 regulates the balance between self-renewal and differentiation in human embryonic stem cells. Nature Cell Biology, 13(6), 652-661. https://doi.org/10.1038/ncb2246

LSD1 regulates the balance between self-renewal and differentiation in human embryonic stem cells. / Adamo, Antonio; Sesé, Borja; Boue, Stephanie; Castaño, Julio; Paramonov, Ida; Barrero, Maria J.; Belmonte, Juan Carlos Izpisua.

In: Nature Cell Biology, Vol. 13, No. 6, 06.2011, p. 652-661.

Research output: Contribution to journalArticle

Adamo, A, Sesé, B, Boue, S, Castaño, J, Paramonov, I, Barrero, MJ & Belmonte, JCI 2011, 'LSD1 regulates the balance between self-renewal and differentiation in human embryonic stem cells', Nature Cell Biology, vol. 13, no. 6, pp. 652-661. https://doi.org/10.1038/ncb2246
Adamo, Antonio ; Sesé, Borja ; Boue, Stephanie ; Castaño, Julio ; Paramonov, Ida ; Barrero, Maria J. ; Belmonte, Juan Carlos Izpisua. / LSD1 regulates the balance between self-renewal and differentiation in human embryonic stem cells. In: Nature Cell Biology. 2011 ; Vol. 13, No. 6. pp. 652-661.
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