Abstract
Coefficient of kinetic friction (μ) between rabbit visceral and parietal pleura, sliding in vitro at physiological velocities and load, increases markedly after blotting mesothelial surface with filter paper; this increase is only partially reduced by wetting blotted mesothelium with Ringer solution. Given that mesothelial surface is covered by a thick coat with sialomucin and hyaluronan, we tested whether addition of sialomucin or hyaluronan solution after blotting lowers μ more than Ringer alone. Actually, these macromolecules lowered μ more than Ringer, so that μ was no longer significantly higher than its preblotting value. Moreover, Ringer addition, after washout of macromolecule solution, increased μ, in line with their dilution. These findings indicate that mesothelial blotting removes part of these molecules from the coat covering mesothelial surface, and their relevance for pleural lubrication. Transmission electron micrographs of pleural specimens after mesothelial blotting showed that microvilli were partially or largely removed from mesothelium, consistent with a substantial loss of macromolecules normally entrapped among them.
| Original language | English |
|---|---|
| Pages (from-to) | 34-39 |
| Number of pages | 6 |
| Journal | Respiratory Physiology and Neurobiology |
| Volume | 180 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 15 2012 |
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Keywords
- Friction coefficient
- Hyaluronan
- Microvilli
- Phospholipids
- Pleural lubrication
- Sialomucin
ASJC Scopus subject areas
- Physiology
- Pulmonary and Respiratory Medicine
- Neuroscience(all)
Cite this
Lubricating effect of sialomucin and hyaluronan on pleural mesothelium. / Bodega, Francesca; Pecchiari, Matteo; Sironi, Chiara; Porta, Cristina; Arnaboldi, Francesca; Barajon, Isabella; Agostoni, Emilio.
In: Respiratory Physiology and Neurobiology, Vol. 180, No. 1, 15.01.2012, p. 34-39.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Lubricating effect of sialomucin and hyaluronan on pleural mesothelium
AU - Bodega, Francesca
AU - Pecchiari, Matteo
AU - Sironi, Chiara
AU - Porta, Cristina
AU - Arnaboldi, Francesca
AU - Barajon, Isabella
AU - Agostoni, Emilio
PY - 2012/1/15
Y1 - 2012/1/15
N2 - Coefficient of kinetic friction (μ) between rabbit visceral and parietal pleura, sliding in vitro at physiological velocities and load, increases markedly after blotting mesothelial surface with filter paper; this increase is only partially reduced by wetting blotted mesothelium with Ringer solution. Given that mesothelial surface is covered by a thick coat with sialomucin and hyaluronan, we tested whether addition of sialomucin or hyaluronan solution after blotting lowers μ more than Ringer alone. Actually, these macromolecules lowered μ more than Ringer, so that μ was no longer significantly higher than its preblotting value. Moreover, Ringer addition, after washout of macromolecule solution, increased μ, in line with their dilution. These findings indicate that mesothelial blotting removes part of these molecules from the coat covering mesothelial surface, and their relevance for pleural lubrication. Transmission electron micrographs of pleural specimens after mesothelial blotting showed that microvilli were partially or largely removed from mesothelium, consistent with a substantial loss of macromolecules normally entrapped among them.
AB - Coefficient of kinetic friction (μ) between rabbit visceral and parietal pleura, sliding in vitro at physiological velocities and load, increases markedly after blotting mesothelial surface with filter paper; this increase is only partially reduced by wetting blotted mesothelium with Ringer solution. Given that mesothelial surface is covered by a thick coat with sialomucin and hyaluronan, we tested whether addition of sialomucin or hyaluronan solution after blotting lowers μ more than Ringer alone. Actually, these macromolecules lowered μ more than Ringer, so that μ was no longer significantly higher than its preblotting value. Moreover, Ringer addition, after washout of macromolecule solution, increased μ, in line with their dilution. These findings indicate that mesothelial blotting removes part of these molecules from the coat covering mesothelial surface, and their relevance for pleural lubrication. Transmission electron micrographs of pleural specimens after mesothelial blotting showed that microvilli were partially or largely removed from mesothelium, consistent with a substantial loss of macromolecules normally entrapped among them.
KW - Friction coefficient
KW - Hyaluronan
KW - Microvilli
KW - Phospholipids
KW - Pleural lubrication
KW - Sialomucin
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UR - http://www.scopus.com/inward/citedby.url?scp=83555176351&partnerID=8YFLogxK
U2 - 10.1016/j.resp.2011.10.006
DO - 10.1016/j.resp.2011.10.006
M3 - Article
C2 - 22019600
AN - SCOPUS:83555176351
VL - 180
SP - 34
EP - 39
JO - Respiratory Physiology and Neurobiology
JF - Respiratory Physiology and Neurobiology
SN - 1569-9048
IS - 1
ER -